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细胞分析试剂盒
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中文名称

PicoProbe™苏氨酸测定试剂盒(荧光法)

英文名字
PicoProbe™ Threonine Assay Kit (Fluorometric)
供应商
Biovision
产品货号
K463-100
产品报价
¥询价/100assays
产品说明书
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产品新闻
背景资料
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产品描述
Threonine is one of the 20 naturally-occuring amino acids used by all organisms in the biosynthesis of proteins and is an essential dietary amino acid for humans. Threonine residues in proteins are subject to post-translational phosphorylation, a critical regulatory and signal transduction mechanism. In addition to its role as a protein building block, threonine can serve as a precursor to other amino acids and metabolites, including glycine, isoleucine and acetyl-coenzyme A. Recent research has shown that threonine is a vital nutrient for embryonic stem cells and threonine metabolism (mediated by the enzyme threonine dehydrogenase) is required for unlimited self-renewal and maintenance of stem cell pluripotency. Dietary threonine deficiency, while rare, can result in breakdown of the intestinal mucosal lining (eventually triggering inflammatory bowel disorders), immunosuppression and a multitude of neuropsychiatric symptoms. BioVision’s Threonine Assay Kit allows for highly sensitive quantification of L-Threonine levels in biological fluids and tissues. The assay is based on the selective, NAD+-coupled enzymatic metabolism of threonine, yielding an oxidized intermediate and NADH. A developer enzyme mixture utilizes the NADH generated to convert PicoProbe™ into a stable fluorophore (Ex/Em = 538/587 nm). The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect threonine levels down to 2 μM in samples
产品特点
• Simple, rapid & convenient assay • Limit of detection: 2 µM • Very specific, does not interact with other standard and non-standard amino acids
保存建议
-20
其他
BioVision的公司总部在美丽的旧金山湾地区,它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品,以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理,艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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