- 中文名称
PPDK（丙酮酸，磷酸二激酶）活性测定试剂盒（荧光/比色法）
- 英文名字
- PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric)
- 供应商
- Biovision
- 产品货号
- K456-100
- 产品报价
- ￥询价/100assays
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868.
- 产品新闻
- 背景资料
- 待更新
- 产品描述
- Pyruvate, phosphate dikinase, PPDK, (E.C. 2.7.9.1) is found in microorganisms and plants where it catalyzes reversible interconversion of AMP, PPi and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate. PPDK plays an important role in plant photosynthesis and glycolytic pathway of endobiotic protozoa and bacteria where pyruvate, phosphate dikinase (PK) is absent; it functions in the process of ATP biosynthesis. In plants, PPDK is a pivotal regulator of the C4 photosynthetic cycle and the key limiting factor for maintaining photosynthesis at low temperature. It catalyzes the regeneration of PEP as the primary acceptor of atmospheric CO2 from pyruvate, ATP and phosphate. In addition, PPDK is also present in C3 leaves where it confers a similar light/dark regulation in chloroplasts, and in wheat leaves in an amount comparable to that found in seeds. Since PPDK is not found in vertebrates, finding its inhibitors may be useful for treatment of infections caused by anaerobic protists that depend on pyrophosphate-dependent glycolysis. Specific inhibition of PPDK is expected to inhibit Giardia and other anaerobic protists such as Entamoeba histolytica and Trichomonas vaginalis without harming their mammalian hosts. BioVision’s Pyruvate, phosphate dikinase, (PPDK) Assay Kit provides a simple and rapid test for measuring specific activity of PPDK in bacterial, protozoan and plant lysates as well as protein preps. We utilize the ability of PPDK to interconvert the substrate into intermediate product detected by proprietary enzyme mix and probe. Generated stable product can be quantified by either colorimetric or fluorometric readout. The assay is simple to perform, high-throughput adaptable and a fluorometric reaction can detect as low as of 0.006 U of PPDK activity in a single well. We provide sufficient reagents for 100 fluorometric or colorimetric assays.
- 产品特点
- • Simple procedure; takes ~ less than 60 minutes
• Fast and convenient
- 保存建议
- -20
- 其他
- BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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