oncentrations of uridine and cytidine analogues (402 µM +/- 252) compared to normal cells (83 µM +/- 133), to support the increased demand for nucleotides in mitosis in actively dividing cells. BioVision’s Cytidine Deaminase Activity Assay Kit uses CDA to convert cytidine to the uridine and NH3, as intermediates. The intermediate products then react with a proprietary Reaction Mix to generate a stable fluorophore that can be detected fluorometrically (Ex/Em= 410/470 nm). The kit is suitable for measuring CDA activity as low as 1 µUnit (1 pmole/min/well) in biological samples
产品特点
• Rapid, and convenient • The kit measures total Activity of Cytidine Deaminase with limit of quantification 1 µU recombinant Cytidine Deaminase. Range of the assay: 10-100 µU.
