- 中文名称
EZClick™唾液酸（ManAz）修饰糖蛋白测定试剂盒（FACS /显微镜，绿色荧光）
- 英文名字
- EZClick™ Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
- 供应商
- Biovision
- 产品货号
- K441-100
- 产品报价
- ￥询价/100assays
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868.
- 产品新闻
- 背景资料
- 待更新
- 产品描述
- Glycans are vital components of glycoproteins, glycolipids, and proteoglycans in all domains of life. Glycosylation occurs co- or post-translationally on >50% of eukaryotic proteins resulting in membrane-associated, intracellular, or secreted glycoproteins that are crucial in cellular processes, protein bioactivity and metabolic turnover. Intracellular glycans mediate protein folding, stability, and trafficking while at the cell surface, they participate in recognition, cell-cell interactions involved in adhesion, migration, and embryonic development; host-pathogen interactions critical for bacterial and viral infections; and initiation of immune response. Aberrant glycosylation profiles correlate with inflammation and are universal feature of cancer, with sialic acids playing an especially prominent role as tumor associated carbohydrate antigens (TACAs). Altered sialylation of tumor cell surfaces is associated with several critical malignant properties that include invasiveness and metastatic potential suggesting its implication in clinical diagnosis. Since glycoproteins are not directly encoded in the genome, methods of characterization and analyses of glycoproteins are of great interest. Thus BioVision offers EZClickTM Sialic Acid (ManAz) Modified Glycoprotein Assay Kit, a highly specific, simple and robust method for labeling and detection of N-linked glycosylation of cell surface proteins. We use a modified mannosamine precursor that is fed directly into the cells, converted to sialic acid by the sialic acid biosynthetic machinery, and transported to the Golgi apparatus for glycan elaboration. Followed by click reaction with alkyne-containing dye, this system offers a powerful method for imaging the localization, trafficking, and dynamics of glycans, or detection by FACS for quantitative studies. Labeled Glycoproteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-alkyne or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format
- 产品特点
- Simple, fast, does not require lengthy incubation times
- 保存建议
- -20
- 其他
- BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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