hat of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. BioVision’s ER-beta ELISA kit is a sandwich ELISA assay for the quantitative measurement of human ER-beta in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human ER-betacaptured from the samples.
产品特点
Easy, convenient and time-saving method to measure the level of ER-beta in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 0.313-20 ng/ml
Sensitivity: < 0.188 ng/ml
