The BioVision’s Mouse IgG ELISA Kit is engineered for precise quantification of total mouse IgG in serum, ascites, and hybridoma cell culture supernatant samples. A polyclonal antibody specific for mouse IgG has been pre-coated onto the 96-well microtiter plate to capture mouse IgG in standards and biological samples. TMB substrate is used to detect HRP conjugated to detection antibody specific for mouse IgG. TMB is converted by HRP to a blue color product that changes into yellow color after adding stop solution. The intensity of the color is proportional to the amount of Mouse IgG in samples and can be measured spectrophotometrically at 450 nm.
产品特点
Easy, convenient and time-saving method to qunatify mouse IgG in biological fluids without its purification.
Detection Range: 1 – 100 ng/ml
