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细胞分析试剂盒
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中文名称

磷脂酶A2活性测定试剂盒(荧光)

英文名字
Phospholipase A2 Activity Assay Kit (Fluorometric)
供应商
Biovision
产品货号
K400-100
产品报价
¥询价/100assays
产品说明书
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产品新闻
背景资料
待更新
产品描述
Phospholipase(s) A2 (PLA2s, EC 3.1.1.4) are a family of enzymes that catalyze the hydrolysis an acyl ester bond in the sn-2 position of a glycerophospholipid to release a lysophospholipid and a free fatty acid. Members of the PLA2 superfamily can be divided into three major subtypes based upon their size and cation dependence. Two of the subtypes, the low molecular weight secretory phospholipase A2 enzymes (sPLA2) and the high molecular weight cytosolic enzymes (cPLA2), are characterized by their requirement of Ca2+ for catalytic activity. The 14 kDa sPLA2 enzymes are known to play an important role in the digestion of dietary phospholipids, host defense against bacterial infections as well as production of eicosanoids. The 85 kDa cPLA2 enzymes play an important role in inflammatory disorders by releasing arachidonic acid from membrane phospholipids for production of various eicosanoids. The third subtype, the high molecular weight calcium-independent phospholipases A2 (iPLA2), are intracellular forms of the enzyme that have been recently implicated in functions such as phospholipid remodeling, cell proliferation and fat catabolism. BioVision’s Phospholipase A2 Activity Assay Kit provides a quick, sensitive and simple way for measuring PLA2 activity in various biological samples. In this assay, active PLA2 cleaves a synthetic thiophospholipid, producing a lysothiophospholipid which reacts with a fluorogenic probe to produce a highly fluorescent product detectable in the visible range (Ex/Em= 388/513 nm). The assay is simple to perform, high-throughput adaptable and can detect less than 0.1 mU of PLA2 activity
产品特点
• Simple, rapid & convenient • The assay can detect total phospholipase A2 (cytosolic and secretory). Activity less than 0.5 mUin various sample types.
保存建议
-20
其他
BioVision的公司总部在美丽的旧金山湾地区,它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品,以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理,艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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