Acyl-CoA Synthetase (ACS) also known as Acyl-CoA ligase, is a ubiquitous enzyme that catalyzes the reaction of free fatty acids and Coenzyme A (CoA) to generate Fatty Acyl-CoAs through an ATP-dependent mechanism. The incorporation of this CoA moiety generates a thio-ester bond that effectively activates the fatty acid for downstream biological processes. Unlike their fatty acid counterparts, Fatty acyl-CoAs also serve as precursors of cellular lipids. Dysregulation of the various isoforms of ACS have been implicated in a number of metabolic diseases, including Non-Alcoholic Fatty Liver Disease (NAFLD) and Hepatic Fibrosis. ACS also plays a role in regulating apoptosis. BioVision’s Acyl-CoA Synthetase Fluorometric assay kit provides a rapid, sensitive and straightforward way to measure ACS activity in various samples. In the assay, acyl-CoA produced by ACS activity is metabolized by the Enzyme Mix, Developer Mix and Converter Mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured with excitation at 535 nm and emission at 587 nm. This assay can detect ACS activity as low as 5 mU/µl in samples.
