- 中文名称
PCR-Legionella spp Plus检测试剂盒
- 英文名字
- PCR-Legionella spp Plus Detection Kit
- 供应商
- Biovision
- 产品货号
- K1451-96
- 产品报价
- ￥询价/96Rxns
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868.
- 产品新闻
- 背景资料
- 待更新
- 产品描述
- PCR-Legionella spp Plus Detection Kit (Legionella spp & Legionella pneumophila) is an ideal tool for an fast and reliable Amplification and Detection of specific DNA fragments from Legionella spp and/or Legionella pneumophila by the real-time PCR method (Multiplex PCR). The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The probe for the detection of target gene is labelled with the FAM (Legionella spp) and HEX fluorochrome (L. pneumophila), whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome.
In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
- 产品特点
- Fast: results in less than 24 hours with minimal handling., Inclusivity: Primers and probes have been tested satisfactorily in 14 strains of L. pneumophila and 30 species of the genus Legionella., Amplification limit: Legionella spp: 5 UG per reaction. L. pneumophila: 100 UG per reaction., Detection: probe labelled with fluorescent dyes– Legionella: FAM-BHQ1a; L. pneumophila: HEXBHQ1; IAC:CY5-BHQ2., Quantification limit: Legionella spp: 50 UG/L. L. pneumophila: 1000 UG/L., Exclusivity: No cross-reactions with other frequent bacterial agents have been observed., Simultaneously detect and differentiate Legionella spp. and L. pneumophila in the same tube, Reliable and highly specific Results., Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers, Quantification Dynamic range: 5 logs, Easy and Ready-to-use Kit.
- 保存建议
- -20
- 其他
- BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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