当特异性地制备用于体内功能测定时，抗体在体外和体内阻断I型IFN受体信号传导，而不消耗携带IFNAR 1的细胞。该抗体通过用编码鼠IFNAR1的胞外结构域的质粒对IFNAR1敲除小鼠进行体内遗传免疫来产生。IFNAR1和IFNAR2在几乎所有细胞上共表达，并构成结合所有I型IFN（IFN α和IFN β）的异二聚体受体。I型IFN是已经显示在体内促进抗病毒、抗微生物、抗肿瘤和自身免疫应答的细胞因子家族。 抗小鼠IFNAR 1（MAR 1 - 5A 3）体内抗体-超低内毒素该产品同种型对照：Mouse IgG1 Isotype Control for In Vivo - Ultra Low Endotoxin [HKSP] (ICH2247UL) 缓冲液：ICH3001-100ml，nICH3002-100ml，nICH3003-100ml， 浓度：≥ 2.0 mg/ml 纯度：>98% by SDS-PAGE and HPLC 内毒素：≤ 0.5 EU/mg as determined by the LAL method 制剂：0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
应用类型
Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, ELISA
免疫原
This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.
