- 中文名称
Isoprostane (8-iso-PGF2a) Serum, Tissue ELISA Kit, BioAssay
- 英文名字
- Isoprostane (8-iso-PGF2a) Serum, Tissue ELISA Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- I9050
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of a representative isoprostane, 15-isoprostane F2t (also known as 8-epi-PGF2 or 8-iso-PGF2) Levels of 15-isoprostane F2t in biological fluids have been shown to be useful for assessment of oxidant stress in vivo. 15- isoprostane F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, andcarcinogenesis.This kit can be used for the quantification of free 15-isoprostane F2t in urine, plasma, serum or tissue samples following solid phase extraction of the isoprostane-containing fraction. Instructions are also provided for the quantification of total 15-isoprostane F2t following hydrolysis of phospholipids.Principle:This kit is a competitive ELISA for determining levels of 15-isoprostane F2t in biological samples. Briefly, 15-isoprostane F2t in the samples or standards competes with 15-isoprostane F2t conjugated to HRP (HRP) for binding to a polyclonal antibody specific for 15-isoprostane F2t coated on the microplate. The HRP activity results in color development when substrate is added, with the intensity of the color proportional to the amount of 15-isoprostane F2t bound and inversely proportional to the amount of unconjugated 15-isoprostane F2t the samples or standards.Sensitivity: 0.1ng/mlDynamic Range: 0.1-1.0ng/mlPerformance Characteristics:Crossreactivity at 50% B/B0:15-isoprostane F2t 100.0%9a,11b-Prostaglandin F2a 4.1%13,14-Dihydro-15-KETO-F2a 3.0%9b,11a-Prostaglandin F2a <0.01%Prostaglandin F2a <0.01%6-KETO-Prostaglandin F2a <0.01% Prostaglandin E2 <0.01%Prostaglandin D2 <0.01%Arachidonic Acid <0.01%Kit Components:I9050A: Microtiter Plate 1x96wellsI9050B: 15-isoprostane F2t Standard 2x60ulI9050C: Wash Buffer (5X) 1x40mlI9050D: Dilution Buffer (5X) 1x100mlI9050E: Substrate 1x25mlI9050F: 15-isoprostane F2t (HRP)1x100ulStorage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Isoprostane (8-iso-PGF2a) Serum, Tissue ELISA Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Isoprostane (8-iso-PGF2a) Serum, Tissue ELISA Kit, BioAssay产品后将其置于4°C Do Not Freeze保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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