Isocitric acid (HOOC-CHOH-CH(-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and a-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to a-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa. Isocitrate BioAssay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In the assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a Lmax of 450nm. The Isocitrate Assay Kit can detect 1-20nmoles (~20uM) of isocitrate in various samples.Kit Components:1. Isocitrate Assay Buffer: 1x25ml 2. Isocitrate Enzyme Mix: 1x200ul 3. Substrate Mix: lyophilized 4. Isocitrate Standard (100mM), 1x100ulStorage and Stability:Store kit at -20°C. Protect from light. Allow Assay Buffer to warm to room temperature before use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
