- 中文名称
Iron Assay Kit, BioAssay
- 英文名字
- Iron Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- I8614-83
- 产品报价
- ￥1/250Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with blood loss, increased destruction of red blood cells (e.g. hemorrhage) or decreased blood cell survival, acute hepatitis, certain sideroachrestic anemias, ingestion of iron-rich diets, defects in iron storage (e.g. pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand on iron storage as during normal pregnancy.Simple, direct and automation-ready procedures for measuring iron concentrations find wide applications in research, drug discovery and environmental monitoring. Iron assay kit is designed to measure total iron directly in serum without any pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe2+. Fe3+ in the sample is reduced to Fe2+, thus allowing the assay for total iron concentration. The intensity of the color, measured at 590nm, is directly proportional to the iron concentration in the sample.Key Features:Sensitive and accurate. Linear detection range 27ug/dL (4.8uM) to 1,000ug/dL (179uM) iron in 96-well plate assay.Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day.Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples.Applications:Direct Assays: iron in biological samples (e.g. serum).Drug Discovery/Pharmacology: effects of drugs on iron metabolism.Environmental Monitoring: iron in soil extracts, mineralized samples.Kit Contents: (250 tests in 96-well plates)Reagent A: 50ml Reagent B: 4ml Reagent C: 4mlIron Standard: 1ml 10 mg/dL Fe2+Storage conditions. The kit is shipped at room temperature. Store Reagent A at room temperature and other reagents at 4 °C. Shelf life: 12 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Note: (1). Iron chelators (e.g. EDTA) interfere with this assay and should be avoided in sample preparation. (2). Serum or plasma samples should be clear and free of precipitates or turbidity. If not, centrifuge or filter to clarify samples prior to assay. (3).This kit can be applied to measure Fe2+ (vs. total iron) content. Prepare Working Reagent by mixing 20 vol of Reagent A, 1 vol of water and 1 vol Reagent C (no reductant in the Working Reagent). The procedure is the same as described for the total iron assay.Procedure using 96-well plate:1. Standards. Prepare 400ul 1000 ug/dL Premix by mixing 40ul 10mg/dL standard and 360ul distilled water. Dilute standards as follows. No Premix+H2O Vol (ul) Fe (ug/dL)1 100ul+0ul 100 10002 80ul+20ul 100 8003 60ul+40ul 100 6004 40ul+60ul 100 4005 30ul+70ul 100 3006 20ul+80ul 100 2007 10ul+90ul 100 1008 0ul+100ul 100 0Transfer 50ul diluted standards and 50ul sample into a clear flat bottom 96-well plate. For serum/plasma samples, it is recommended to run a sample blank (i.e. a 50ul sample in a separate well).2. Prepare enough Working Reagent by mixing 20 volumes of Reagent A, 1 volume Reagent B and 1 volume Reagent C. Fresh reconstitution is recommended. Equilibrate to room temperature before assay. Add 200ul Working Reagent to Standards and Samples wells. (For serum/plasma samples which require a Sample Blank Control, add 200ul Reagent A to the Sample Blank wells). Tap plate to mix.3. Incubate 40 min at room temperature and read optical density at 510-630nm (peak absorbance at 590nm).Procedure using cuvette:1. Prepare standards as in 96-well assay. Set up centrifuge tubes labeled Standards and Samples. Transfer 250ul standards and samples to tubes.2. Add 1000ul Working Reagent to all tubes. Mix by vortexing. Incubate 40 min at room temperature.3. Transfer to cuvettes and read OD at 590nm (510nm-630nm).Calculation:Subtract OD of “0 ug/dL Fe” from all other standard OD values and plot the OD against standard iron concentrations. Determine the slope using linear regression fitting. Iron concentration of the sample is calculated as[Iron]=ODSAMPLE-ODBLANKSlope (ug/dL)Where ODBLANK is OD values of the water blank (Standard #8), or Sample Blank, if a sample blank is used (e.g. serum or plasma). Typical serum iron values: 70-180 ug/dL.Conversions: 1 mg/dL Fe equals 179uM, 0.001% or 10 ppm.Materials Required, But Not Provided:Pipeting devices and accessories.Procedure using 96-well plate:Clear bottom 96-well plates (e.g. Corning Costar) and plate reader.Procedure using cuvette:Cuvets and spectrophotometer for measuring OD at 510-630nm.Examples:Mouse serum, fetal bovine serum, and goat serum were assayed using the 96-well plate assay protocol. The iron concentrations were 173 ± 2 (n=4), 149 ± 1 (n=4), 88 ± 2 μg/dL (n=4), respectively. Coefficient of variance < 2%.
- 产品特点
- 针对Iron Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Iron Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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