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中文名称

Interleukin 13 Receptor Alpha 2, Human (IL-13 Ra2) BioAssay ELISA Kit

英文名字
Interleukin 13 Receptor Alpha 2, Human (IL-13 Ra2) BioAssay ELISA Kit
供应商
USBiological
产品货号
I8435-09B
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
This assay applies the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific to IL-13 Ra2 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-13 Ra2 and incubated. IL-13 Ra2 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be sandwiched by biotin conjugate. After washing away any unbound substances. In order to quantitate the amount of IL-13 Ra2 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13 Ra2, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The color development is stopped and the intensity of the color is measured.In order to measure the concentration of IL-13 Ra2 in the samples, this package of reagent includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-13 Ra2 concentration (ng/ml). The concentration of IL-13 Ra2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: <10ng/mlAssay Range: 10-500ng/mlKit Components:I8435-09B1. IL-13 Ra2 Microtiter Plate 1x96 wellsI8435-09B2. Biotin Conjugate, 1x6mlI8435-09B3. Avidin Conjugate, 1x15ml I8435-09B4. IL-13 Ra2 STANDARD 2x0.5ug I8435-09B5. CALIBRATOR DILUENT I, 1x25ml For serum/plasma testing.I8435-09B6. CALIBRATOR DILUENT II, 1x25ml For cell culture supernatant testing.I8435-09B7. WASH BUFFER (20X), 1x60mlI8435-09B8. SUBSTRATE A, 1x10mlI8435-09B9. SUBSTRATE B, 1x10mlI8435-09B10. STOP SOLUTION, 1x15mlStorage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
产品特点
针对Interleukin 13 Receptor Alpha 2, Human (IL-13 Ra2) BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Interleukin 13 Receptor Alpha 2, Human (IL-13 Ra2) BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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