- 中文名称
Interleukin 10, Human (IL-10), BioAssay ELISpot Kit
- 英文名字
- Interleukin 10, Human (IL-10), BioAssay ELISpot Kit
- 供应商
- USBiological
- 产品货号
- I8432-06R
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use:This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-10.Principle:Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human IL-10 binds to antibody coated ELISpot plate. ELISpot plate coated with human IL-10 Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-10.Background:The mature human IL-10 is a protein with 160 amino acids and the functional IL-10 is a homodimer. IL-10 is expressed by a variety of cells, including mouse T cells (TH1, TH2, and Tr1 subsets), B cells derived from peripheral blood, tonsils or spleen, Epstein–Barr virus (EBV)-transformed B cell lines, Burkitt’s lymphoma, AIDS lymphomas, monocytes, placental trophoblasts, bronchial epithelial cells, and certain tumor cells including melanomas and carcinomas of various origin.IL-10 plays an important role in inflammatory and immune responses. The biological activities of IL-10 include both immunosuppressive and immuno-stimulatory effects. For example, IL-10 can suppress the production of pro-inflammatory cytokines by monocytes and neutrophils, and down regulates the expression of activating and co-stimulatory molecules on monocytes and dendritic cells. IL-10 also can improve the growth of B cells and mast cells, and inhibit or enhance the activities of T cells depending on their activation conditions. The different polymorphisms in the IL-10 gene promoter have been demonstrated the association with both SIDS (sudden infant death syndrome) and sudden unexpected death due to infection.Based on the functions of IL-10, it has been suggested that an IL-10 antagonist can be applied to boost anti-viral immunity against viruses such as EBV and that the IL-10 molecule itself may be used as an anti-inflammatory reagent.Kit Components:A. Microtiter Plates 1x96wells PVDF-bottom Immunospot plates pre-coated with mouse anti-human IL-8 monoclonal antibody. B. Positive Control 1x1 vial Supplied as a lyophilized recombinant human IL-10. Reconstitute with 250 ul Cell Culture Media before use. Use in 1 hour. C. Wash Buffer (20x)1x 60ml. Add 1 volume of Wash Buffer (20x) to 19 volume of ddH2O. Use in 1 week. Stored at room temperature.D. IL-10 (Biotin) 1x11ml Biotinylated mouse anti-human IL-10 monoclonal antibody. Ready to use. E. Streptavidin (AP)(100x)1x120ul. Add 1 volume of Concentrated Streptavidin to 100 volumes of F. (Streptavidin (AP) Diluent) before use. Use in 1 month. Stored at 4°C. F. Streptavidin (AP) Diluent 1x11ml. Ready to use. G. BCIP/NBT Substrate Solution 1x11ml . Ready to use.Storage and Stability: Store other components at 4ºC. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interleukin 10, Human (IL-10), BioAssay ELISpot Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin 10, Human (IL-10), BioAssay ELISpot Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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