- 中文名称
Interleukin 4, Human (IL-4) BioAssay ELISpot Kit
- 英文名字
- Interleukin 4, Human (IL-4) BioAssay ELISpot Kit
- 供应商
- USBiological
- 产品货号
- I8426-01A
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use:This 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-4.Principle:Add stimulated cells or cells and stimulant to the wells and incubate at 37°C in CO2 incubator for a specified period. Secreted human IL-4 binds to antibody coated microtiter plate.microtiter plate coated with human IL-4 Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-4. Substrate Solution is added. A colored precipitate forms and appears as spots at the sites of human IL-4 secreting location. Each individual spot representing an individual human IL-4 secreting cell.Background:Interleukin 4 (IL-4) was initially characterized as a B cell stimulatory factor (BSF-1) (1) and B-cell differentiation factor (BCDFγ) (2). It was subsequently revealed that IL-4 is a pleiotropic cytokine with multiple immune response modulating functions on diverse cell types including T cells, monocytes, macrophages, mast cells, fibroblasts, endothelial cells, osteoblasts, keratinocytes, hepatocytes and astrocytes (3-8). IL- 4 is produced by CD4+ TH0 and TH2 cells (9,10), fetal thymocytes (11), CD8+ T cells (12), mast cells (13) and basophils (14).Human and mouse cDNAs for IL-4 encode precursor proteins containing 153 and 140 amino acid residues, respectively. The signal peptides from the precursors are cleaved to yield mature proteins of 129 amino acid residues (human) and 120 amino acid residues (mouse) (17, 18). Both human and mouse proteins have multiple potential glycosylation sites and six cysteines that are all involved in intro-molecular disulfide bridges.IL- 4 exhibits approximately 25% amino acid sequence homology to IL-13 that shares a number of biological functions with IL-4 (19). The human and mouse IL-4 gene, each composed of four exons and three introns, have been localized to human chromosome 5q23-31 and mouse chromosome 11 in tandem with genes for IL-3, IL-5, IL-9, IL-13 and GM-CSF (20, 21). IL-4 is an anti-inflammatory cytokine that exhibits multiple immuno-modulation functions on a variety of cell types, including T cells, B cells, monocytes, neutrophils, hematopoietic progenitors, fibroblasts, endothelial cells, and epithelial cells (15, 16).The diverse effects exhibited by IL-4 in vitro suggest that it may play a central role in the modulation of immune and inflammatory responses in vivo. It was reported that introduction of malignant tumor cells transfected with the gene for IL-4 and producing IL-4 in athymic mice can block tumor formation by other transplantable tumor lines in vivo. Since the anti-tumor activity of IL-4 is evident in these athymic mice, IL-4-mediated host-defense responses other than T cell immunity must be involved (22). It was reported that IL-4 is important for protective immunity in parasitic nematode-infected mice, since IL-4 or IL-4R antibodies can blocked the polyclonal IgE response and abrogated protective immunity to the infection (23). In contrast to results obtained with the nematode-infected mice, endogenous IL-4 was reported to inhibit protective immunity in mice infected with the protozoan Leishmania major (24, 25). Clearly, much more work is required to unravel the complex network of IL-4-dependent processes in order to utilize IL-4 successfully in immunotherapy (16).Kit Components:I8426-01A1: Microtiter Plate 1x96wells. PVDF-bottom Immunospot plates pre-coated with mouse anti-human IL-4 monoclonal antibody.I8426-01A2: Positive Control 1x1vial. Supplied as a lyophilized recombinant human IL-4. Reconstitute with 250ul Cell Culture Media before use. Use in 1 hour. The final concentration is 8ng/ml.I8426-01A3: Wash Buffer (20X) 1x60ml. Add 1 volume of Wash Buffer (20X) to 19 volume of ddH2O. Use in 1 week. If the concentrate is stored ar 4ºC, crystals may form. Allow to reach RT before reconstitution.I8426-01A4: Mab Detection Antibody (Biotin) 1x11ml. Biotinylated mouse anti-human IL-4 monoclonal antibody. Ready to use. I8426-01A5: Streptavidin (AP) (100X) 1x120ul. Add 1 volume of I8426-01A5 Streptavidin (AP)(100X) to 100 volumes of I8426-01A6 Streptavidin (AP) Diluent before use. Use in 1 month.I8426-01A6: Streptavidin (AP) Diluent 1x11ml. Ready to use. I8426-01A7: BCIP/NBT Substrate Solution 1x11ml. Ready to use.Storage and Stability: Store all components at 4ºC. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interleukin 4, Human (IL-4) BioAssay ELISpot Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin 4, Human (IL-4) BioAssay ELISpot Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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