- 中文名称
Interleukin 2, Human (IL-2) BioAssay ELISA Kit
- 英文名字
- Interleukin 2, Human (IL-2) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- I7663-28E
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- This human IL-2 ELISA is a 4.5 hour solid phase immunoassay readily applicable to measure IL-2 levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 2500pg/ml. It showed no cross reactivity with other cytokines tested. This IL-2 ELISA is expected to be effectively used for further investigations into the relationship between IL-2 and the various conditions mentioned.This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody-enzyme specific for IL-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.In order to measure the concentration of IL-2 in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-2 concentration (pg/ml). The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: ≥5.7pg/ml (using Calibrator Diluent I)≥7.1pg/ml (using Calibrator Diluent II)Range: 39-2500pg/mlKit Components:I7663-28E1: Microtiter Plate,1x96 wells. Precoated with anti-human IL-2 Mab*I7663-28E4: IL-2 Standard (5ng/vial), 2x1vial. Supplied as a lyophilized powder containing recombinant human Il-2 in a buffered protein base with preservative.I7663-28E5: Calibrator Diluent I, 1x25ml. For serum/plasma testing. Supplied as animal serum with buffer and preservative.I7663-28E6: Calibrator Diluent II, 1x25ml. For cell culture supernatant testing. Supplied as cell culture medium with calf serum and preservative.I7663-28E7: Wash Buffer (20X), 1x60ml. Supplied as a concentrated solution of buffered surfactant.I7663-28E8: Substrate A, 1x10ml. Supplied as a buffered solution with H2O2.I7663-28E9: Substrate B, 1x10ml. Supplied as a buffered solution wih TMB.I7663-28E10; Stop Solution, 1x14ml. Supplied as 2N Sulphuric Acid (H2SO4). I7663-28E11: IL-2 Pab (Biotin)1x6mlI7663-28E12: Avidin (HRP) 1x12mlStorage and Stability:Store *I7663-28E4 powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interleukin 2, Human (IL-2) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin 2, Human (IL-2) BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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