- 中文名称
Interleukin-1 beta, Human, BioAssay ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta)
- 英文名字
- Interleukin-1 beta, Human, BioAssay ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta)
- 供应商
- USBiological
- 产品货号
- I7663-14
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Interleukin 1 (IL-1) is considered the first of the super-family of regulatory and inflammatory cytokines. 1 There are two distinct IL-1 proteins, Interleukin 1b and Interleukin 1a.Il-1 has a number of alternative names, including lymphocyte activating factor, endogenous pyrogen, catabolin, hemopoietin-1, melanoma growth inhibition factor, and osteoclast activating factor (2, 3). The properties and biological activities of IL-1 have been extensively reviewed. IL-1 is expressed by many cells and has multiple functions including local inflammation. Cells known to express IL-1b include astrocytes (6), adrenal cortical cells, 7 NK cells (8), macrophages and monocytes (9), endothelial cells (10), keratinocytes (16), mega-karyocytes and platelets (11, 12), neurons (13), neutrophils (14). oligodendroglia (6), osteoblasts (5), Schwann cells (6), trophoblasts (8) ,and T cells plus fibroblasts (4).IL-1 has multiple functions including local inflammation. Following bacterial or immunoglobulin ligation of monocyte/macrophage CD14 (the LPS receptor) (17) or CD64 (the IgG receptor), (18) IL-1 can be released into a local environment. Within this environment, IL-1 impacts a number of cells. First, capillary endothelial cells are induced to do two things, one, secrete chemokines such as MCP-1 (19), and two, up-regulate the expression of vascular adhesion molecules such as E-Selectin, ICAM-1 and VCAM-1.20 MCP-1 provides a stimulus for chemotaxis and activates mononuclear cell integrins (21), thus facilitating mononuclear infiltration into an area of early inflammation. IL-1 also induces expression of itself in newly arriving monocytes, thus reinforcing the overall process (22). In terms of other pro-inflammatory molecules, IL-1 apparently is needed for the efficient production of IFN-g. On resident NK cells, IL-1 apparently works in conjunction with macrophage-derived IL-12 to induce IFN-g secretion ( 23), resulting in an IFN-g induced activation of macrophages (24). Finally, IL-1 also induces the expression of MMPs from resident fibroblasts. This can have at least two effects; first extracellular matrix degradation can facilitate monocyte migration, and second, MMPs are known to degrade IL-1b, thus down-modulating the local inflammatory response initiated by IL-1.IL-1 is generally thought of as prototypical pro-inflammatory cytokines (2, 3). The effects of IL-1, however, are not limited to inflammation, as IL-1 has also has been associated with bone formation and re-modeling (5, 25), insulin secretion (26, 27), appetite regulation (28, 29), fever induction, neuronal phenotype development, and IGF/GH physiology (30-32).This IL-1b ELISA is a 3.5 hour solid phase immunoassay readily applicable to measure IL-1b levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 400pg/ml. It showed no cross reactivity with various other cytokines superfamily proteins. This IL-1b ELISA is expected to be effectively used for further investigations into the relationship between IL-1b and various diseases.Principle of the Assay:This IL-1b enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-1b and incubated. IL-1b if present, will bind and become immobilized by the antibody pre-coated on the wells and then be sandwiched by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-1b and other components of the sample. In order to quantitate the amount of IL-1b present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each have a high affinity binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-1b, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm. In order to measure the concentration of IL-1b in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-1b concentration (pg/ml). The concentration of IL-1b in the samples is then determined by comparing the O.D. of the samples to the standard curve. Sensitivity: <2pg/mlRange: 2-400pg/mlKit Components:1. I7663-14A: Microtiter Plate 1x96wellsPre-coated with anti-human IL-8 monoclonal antibody2. I7663-14B: IL-1b Mab (Biotin) 1x7mlAnti-human IL-1b monoclonal antibody conjugated to BiotinSupplied as a liquid.3. I7663-14C: Avidin (HRP) 1x12mlAvidin conjugated to horseradish peroxidaseSupplied as a liquid4. I7663-14D: IL-1b Standard, Recombinant 2x800pgRecombinant human IL-1b in a buffered protein base with preservative.Supplied as a lyophilized powder5. I7663-14E: Calibrator Diluent I 1x25mlSupplied as animal serum with buffer and preservative. For serum/plasma testing6. I7663-14F: Calibrator Diluent II 1x25mlSupplied as celll culture medium with calf serum and preservative. For cell culture supernatant testing7. I7663-14G: Wash Buffer (20X) 1x60mlSupplied as a concentrated solution of buffered surfactant8. I7663-14H: Substrate A 1x10mlSupplied as a buffered solution containing hydrogen peroxide9. I7663-14J: Substrate B 1x10mlSupplied as a buffered solution containing TMB10. I7663-14K: Stop Solution 1x14mlSupplied as a liquid containing 2N sufuric acid Storage and Stability:Store all kit components except I7663-14D at 4°C. Stable for 6 months. Store I7663-14D powder at 4°C and liquid at -20°C (30 days). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interleukin-1 beta, Human, BioAssay ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin-1 beta, Human, BioAssay ELISA Kit (IL-1 beta, Catabolin, IL-1B, IL1F2, IL1-beta)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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