- 中文名称
Interleukin 17 (IL-17) Dual Color, BioAssay ELISpot Kit (Canine) (Interferon gamma, IFN-gamma, IFNg)
- 英文名字
- Interleukin 17 (IL-17) Dual Color, BioAssay ELISpot Kit (Canine) (Interferon gamma, IFN-gamma, IFNg)
- 供应商
- USBiological
- 产品货号
- I7661-65F
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Dual-Color Canine IFN-g/IL-17 ELISpot assay is designed for the simultaneous detection of IFN-g and IL-17 secreting cells at the single cell level and can be used to simultaneously quantitate the frequency of canine IFN-g and IL-17 secreting cells. ELISpot assays are well suited for monitoring immune responses to various treatments and therapies and have been used for the quantitation of antigen-specific CD4+ and/or CD8+ T cell responses. Other methods for assessment of antigen-specific T cell responses, such as chromium release assays with quantitation by limiting dilution, are tedious and require previous in vitro expansion of T cells for several days. These assays typically are not suitable for measuring infrequent T cell responses that occur at less than 1 in 1000. ELISpot assays are highly reproducible and sensitive and can be used to measure responses with frequencies well below 1 in 100,000. ELISpot assays do not require prior in vitro expansion of T cells and are suitable for high-throughput analysis using only small volumes of primary cells. As such, ELISpot assays are useful tools for research in vaccine development and for the monitoring of various clinical trials.Test Principle:The enzyme-linked immunospot (ELISpot) assay was originally developed for the detection of individual B cells secreting antigen-specific antibodies (1, 2). This method has since been adapted for the detection of individual cells secreting specific cytokines or other antigens (3, 4). ELISpot assays employ the quantitative sandwich enzyme-linked immunosorbent assay (ELISA) technique. A monoclonal antibody specific for canine IFN-g and a monoclonal antibody specific for canine IL-17 have been pre-coated onto a PVDF (polyvinylidene difluoride)-backed microplate. Appropriately stimulated cells are pipetted into the wells and the microplate is placed into a humidified 37°C CO2 incubator for a specified period of time. During this incubation period, the immobilized antibodies in the immediate vicinity of the secreting cells bind secreted IFN-g and IL-17. After washing away any cells and unbound substances, a biotinylated polyclonal antibody specific for canine IFN-g and a horseradish peroxidase-conjugated polyclonal antibody specific for canine IL-17 are added to the wells. Following a wash to remove any unbound antibodies, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. After washing the BCIP/NBT from the wells with tap water, an AEC chromogen solution is then added to the wells. A blue-black colored precipitate forms and appears as spots at the sites of cytokine localization, with each individual spot representing an individual IFN-g secreting cell. A red precipitate also forms and appears as spots, with each red spot representing an individual IL-17 secreting cell. The spots can be counted with an ELISpot reader system or using a stereomicroscope.Kit Components:Canine IFN-g/IL-17 Microplate: 1x96-well PVDF-backed microplate coated with a monoclonal antibody specific for canine IFN-g and a monoclonal antibody specific for canine IL-17.Detection Antibody Concentrate A: 150ml of a 120X concentrated solution of biotinylated polyclonal antibody specific for canine IFN-g with preservatives.Detection Antibody Concentrate B: 250ml of a 60X concentrated solution of horseradish peroxidase-conjugated polyclonal antibody specific for canine IL-17 with preservatives.Streptavidin-AP Concentrate A: 150ml of a 120X concentrated solution of Streptavidin conjugated to Alkaline Phosphatase with preservatives.Dilution Buffer: 1x12ml of a buffer for diluting Detection Antibody Concentrates with preservatives.Dilution Buffer: 2x12ml of a buffer for diluting Streptavidin-AP Concentrate A with preservatives.Wash Buffer Concentrate: 50ml of a 10X concentrated solution of a buffered surfactant with preservative.BCIP/NBT Chromogen: 1x12ml of a stabilized mixture of 5-Bromo-4-Chloro-3¢ Indolylphosphate p-Toluidine Salt (BCIP) and Nitro Blue Tetrazolium Chloride (NBT).Canine IFN-g Positive Control: 2ng of recombinant canine IFN-g; lyophilized. 10. Canine IL-17 Positive Control: 2ng of recombinant canine IL-17; lyophilized.AEC Chromogen: 300ml of 2% 3-Amino-9-Ethyl-Carbazole (AEC) in stabilizing buffer.AEC Chromogen Buffer: 12ml of 0.1% H2O2 in Acetate Buffer for diluting AEC Chromogen.Dual-Color ELISpot Schematic: A full-color diagram of the assay principle. Storage and Stability:Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interleukin 17 (IL-17) Dual Color, BioAssay ELISpot Kit (Canine) (Interferon gamma, IFN-gamma, IFNg)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin 17 (IL-17) Dual Color, BioAssay ELISpot Kit (Canine) (Interferon gamma, IFN-gamma, IFNg)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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