- 中文名称
In situ Hybridization Kit BioAssay (ISH)
- 英文名字
- In situ Hybridization Kit BioAssay (ISH)
- 供应商
- USBiological
- 产品货号
- I7643-50
- 产品报价
- ￥23
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- In situ hybridization (ISH) is a powerful method for gene expression analysis. This In situ Hybridization Kit (ISH), BioAssay provides the reagents, including hybridization buffer, NBT/BCIP color development solution and washing buffer solutions, for pre-hybridization, hybridization, and post hybridization treatments. This ISH kit provides researchers a method doing in situ hybridization with non-radioactive probes. Compared with radioactive methods, the sensitivity of this kit is the same while the procedure is simplified. This kit is suitable for digoxigenin labled probes combined with an alkaline phosphatase color development system. Using this kit, all disadvantages of working with radioactive materials can be avoided.Features:• microRNA detection• Non-isotopic, colorimetric detection of gene expression• RNase-free, ISH-optimized reagents• Can be used with either oligonucleotide or ribonucleotide probes• Tested by Digoxigenin labeled oligonucleotide probes• Optimization of ISH assay conditions• ISH detection in tissue section and/or tissue microarray slidesApplications:• Diagnostic determination and clinical decision-making• Gene mutation studies• Protein expression and function analysisKit Components:I7643-50A: Prehybridization Solution, 1x2ml or 1x5mlI7643-50B: Hybridization Solution, 1x2ml or 1x5mlI7643-50C: NBT, 1x120ul or 1x300ulI7643-50D: BCIP, 1x60ul or 1x150ulI7643-50E: SSC Buffer, 20X, 1x20ml or 1x50mlI7643-50F: Alkaline Phosphatase Buffer, 10X, 1x20ml or 1x50mlI7643-50G: anti-Dig Antibody, 1x10ul or 1x25ulI7643-50H: Blocking Solution, 100X, 1x100ul or 1x250ulBuffer Required but Not Included: 1X PBS solution, pH 7.4: 81ml 0.2M Na2HPO4, 19ml 0.2M NaH2PO4, 18g sodium chloride Add DEPC-treated water to 2000ml.Storage and Stability:Store components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Quality Control:In order to ensure the quality of In situ Hybridization Kit (ISH), BioAssay, a pre-test procedure is performed for each lot of the kits. The positive result is detected with a digoxigenin labeled Has-mirRNA 122a cDNA probe on a previous positive frozen tissue panel slide.
- 产品特点
- 针对In situ Hybridization Kit BioAssay (ISH)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到In situ Hybridization Kit BioAssay (ISH)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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