- 中文名称
Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay
- 英文名字
- Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay
- 供应商
- USBiological
- 产品货号
- I7506-05
- 产品报价
- ￥1/50 Slides
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay kit is designed to allow immunohistochemical studies of mouse or rat samples using mouse, rabbit, rat and guinea pig primary antibodies. The kit can be used with all common sample preparation methods including: frozen tissues, paraffin-embedded sections, and cell smears. Unlike most immunostaining systems, in which the anti-mouse secondary antibody causes non-specific staining of endogenous rodent immunoglobulins, the kit uses the a specific method to prevent this unwanted staining. The kit incorporates with a high sensitive horseradish peroxidase (HRP) second antibody to increase sensitivity. And the detection system is faster and cleaner because it uses one step of HRP conjugated to second antibody instead of two steps, as in the LAB-SA method.Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay does not contain biotin or streptavidin, so potential background due to endogenous biotin activity is completely avoided. In addition, a special blocking system is used to eliminate background staining. After a user-supplied primary antibody is incubated with the sample, a HRP-conjugated, affinity-purified secondary antibody is bound to the primary antibody. A DAB chromogen/substrate system then creates an intense brown deposit around the antigen/antibody/enzyme complex in the sample.The kit uses a horseradish peroxidase-conjugated second antibody, and a substrate-chromogen mixture to demonstrate antigen in cells or tissues.The principle of this staining system is as follows:1) Paraffin embedded tissue should be deparaffinized and rehydrated.Endogenous peroxidase activity can be quenched by treating tissue sections with 3% hydrogen peroxide in absolute methanol.2) Non-specific background is eliminated by incubating the tissue section with blocking solution A (I7506-05A), and blocking solution B (I7506-05B).3) The mouse, rabbit, rat or guinea pig primary antibody, for a specific antigen, is incubated on the tissue.4) Incubation is followed by addition of a HRP-conjugated second antibody (I7506-05C), binding to the primary antibody.5) The presence of peroxidase can be revealed by addition of substrate chromogen solution (I7506-05D, I7506-05E & I7506-05F: see protocol).Peroxidase will catalyze the substrate (hydrogen peroxide) and convert the chromogen (DAB) to a brown deposit, which demostrates the location of the antigen.The kit is designed to reveal antigens on mouse or rat tissue or cell samples that have reacted with a user-supplied, primary antibody. Depending on the reactivity of the primary antibody, antigens such as cell surface markers, hormones, cytoskeletal proteins and tumor markers can be detected on properly prepared samples. Frozen or paraffin-embedded tissues, freshly prepared lymphocytes, and fixed cultured cells are the most common preparations. Use for research only.Kit Components:I7506-05A: Blocking Solution A, 1x6ml (ready-to-use).I7506-05B: Blocking Solution B, 1x6ml (ready-to-use).I7506-05C: Secondary Antibody (HRP), 6ml (ready-to-use).I7506-05D: Substrate Buffer (20X), 1x2mlI7506-05E: DAB Chromogen (20X), 1x2mlI7506-05F: 0.6% H2O2 (20X), 1x2mlI7506-05G: Hematoxylin Solution, 1x6ml (ready-to-use)I7506-05H: Mounting Solution, 1x10ml (ready-to-use)Storage and Stability:May be stored at 4°C. Do not freeze. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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