- 中文名称
Immunohistochemistry Detection Kit, Streptavidin HRP, Rabbit, BioAssay
- 英文名字
- Immunohistochemistry Detection Kit, Streptavidin HRP, Rabbit, BioAssay
- 供应商
- USBiological
- 产品货号
- I7506-04
- 产品报价
- ￥1/1000 Slides
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Immunohistochemistry Detection Kit, Streptavidin, Rabbit, BioAssay is designed to reveal antigens that have reacted with a user-supplied primary antibody on human tissue or cell samples. Frozen or paraffin-embedded tissues, freshly prepared lymphocytes, and fixed cultured cells can be used.Streptavidin-Biotin Amplification) has been acknowledged as the premier detection method for IHC, a methodology now widely used in basic research and routine testing, providing enhanced sensitivity and performance in immunohistochemistry (IHC) applications. DAB stained slides can be dehydrated and permanently mounted using mounting medium.The kit is designed to reveal the presence of antigens in human tissue or cell preparations and can be used with all common sample preparation methods, including: frozen tissues, paraffin-embedded sections, and cell smears.After formalin-fixed, paraffin-embedded tissue sections have been deparaffinized in xylene and dehydrated in a graded series of ethanol, an endogenous peroxidase quenching step is performed. This endogenous peroxidase activity can be quenched by either using 3% hydrogen peroxide in methanol. Non-immune serum is used to eliminate non-specific background. After a user-supplied primary antibody has been incubated with a sample, the method uses an enhanced, human-absorbed, biotinylated, affinity purified secondary antibody to react with the primary antibody. Enhanced horseradish peroxidase conjugated streptavidin (HRP-SA) is then bound to the biotinylated secondary antibody. The chromogen is then added, and the peroxidase catalyzes the substrate (hydrogen peroxide) and converts the chromogen to either a brown (DAB) or red (AEC) deposit, which visualizes the location of the antigen. This kit can also be used with automated stainers. Kit Components:I7506-04A: Reagent A, 1x110ml. Ready-to-use serum blocking solution,10% goat non-immune serumI7506-04B: Reagent B. 1x110ml. Ready-to-use biotinylated second antibodyI7506-04C: Reagent C. 1x110ml. Ready-to-use streptavidin-peroxidase (HRP)Storage and Stability:Store all kit components at 4°C. Kit is stable for 6 months at 4°C after receipt.Materials required but not provided:Xylene, ethanol, and absolute methanolDistilled or deionized water30% hydrogen peroxide10mM phosphate-buffered saline (PBS), pH 7.4 or 50mM Tris-buffer saline (TBS), pH 7.8. Optional: 0.05% Tween 20.Mini PAP PenPrimary antibodyHRP Substrate/Chromogen reagents:*AEC Single Solution or DAB KitHematoxylin Mounting Media*This kit does not contain a chromogen. AEC Single Solution or DAB Kit are recommendedto be used with this kit.
- 产品特点
- 针对Immunohistochemistry Detection Kit, Streptavidin HRP, Rabbit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Immunohistochemistry Detection Kit, Streptavidin HRP, Rabbit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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