- 中文名称
IgG3, Mouse, BioAssay ELISA Kit
- 英文名字
- IgG3, Mouse, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- I1904-85T
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Immunoassays using heavy-chain specific antibodies provide for selective, sensitive quantification of mouse immunoglobulins IgG (and subclasses), IgA and IgM, as found circulating in blood or as present in other body fluids, including saliva, milk/colostrum, ascites, tears and mucosa of linings of the gut, respiratory or urigenital tracts.Levels of total IgG (and subclasses), IgA and/or IgM can reveal health status or results of experimental or pathological conditions (e.g., hypo- or hypergammaglobulinemia or acute or chronic infection). Also, measurements of specific antibody levels, in antigen-specific assays, are often best interpreted relative to values of total IgG, IgA, and IgM in the sample and/or individual.The assays are also suitable for quantifying monoclonal antibodies and can be a reliable method for monitoring production and establishing quality control.The quantitative immunoassays measure mouse IgG and subclasses, IgA and IgM with high sensitivity; this allows dilution beyond interference from the sample matrix for samples derived from any of the above specimen types. Expected performance of each kit relative to precision, recovery and linearity of dilution is presented for guidance of use and experimental design.Sample Type:Serum. Plasma, Culture medium and other fluids may be adapted.Sensitivity:25ng/ml; 105 minRange:Assay of IgG3 in stored sera from twenty (20) individual Swiss mice ranged from 1.4-6mg/ml (median = 3.4mg/ml). Each laboratory should determine expected values of its own testing population.Intended Use:The Mouse IgG3 ELISA Kit is an in vitro immunoassay for the quantification of IgG3 circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.Test Principle:The Mouse IgG3 ELISA kit is based on the binding of mouse IgG3 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgG3 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgG3 in samples and control is calculated from a curve of standards containing known concentrations of IgG3.Kit Components:Anti-Mouse IgG3 Microwell Strip Plate: 12X8-well stripsMouse IgG3 Control: 1X0.65mlMouse IgG3 Standard (50ng/ml): 1X0.65mlMouse IgG3 Standard (100ng/ml): 1X0.65mlMouse IgG3 Standard (400ng/ml): 1X0.65mlMouse IgG3 Standard (400ng/ml): 1X0.65mlMouse IgG3 Standard (1000ng/ml): 1X0.65mlAnti-Mouse IgG3 HRP Conjugate (100X): 1X0.15mlSample Diluent Concentrate (20X): 1X10mlWash Solution Concentrate (100X): 1X10mlTMB Substrate: 1X12mlStop Solution: 1X12mlStorage and Stability:Store liquids at -20°C. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对IgG3, Mouse, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到IgG3, Mouse, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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