- 中文名称
Insulin-Like Growth Factor I Receptor, beta, phosphorylated (Tyr1131) BioAssay ELISA Kit (IGF1Rb)
- 英文名字
- Insulin-Like Growth Factor I Receptor, beta, phosphorylated (Tyr1131) BioAssay ELISA Kit (IGF1Rb)
- 供应商
- USBiological
- 产品货号
- I1901
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Insulin-Like Growth Factor I Receptor, beta, phosphorylated (Tyr1131) BioAssay ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of IGF-I receptor b protein when phosphorylated at Tyr1131. A Phospho-IGF-I Receptor b (Tyr1131) Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, phospho-IGFI Receptor b is captured by the coated antibody. Following extensive washing, an IGF-I Receptor Mouse Antibody is added to detect the captured phospho-IGF-I receptor protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for this developed color is proportional to the quantity of IGF-I receptor protein phosphorylated at Tyr1131.Type I insulin-like growth factor receptor (IGF-IR), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation. Insulin receptors (IRs) share significant similarity with IGF-I receptors in both structure and function, including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151). Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.Kit Components:I1901A: Microtiter Strips, 1x96 wellsI1901B: IgG Detection Antibody, 1x11mlI1901C: IgG (HRP), 1x11mlI1901D: Sample Diluent, 1x25mlI1901E: Cell Lysis Buffer (10X), 1x15mlI1901F: Wash Buffer (20X), 1x25mlI1901G: TMB Substrate, 1x11mlI1901H: Stop Solution, 1x11mlStorage and Stability:Store I1903E at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Insulin-Like Growth Factor I Receptor, beta, phosphorylated (Tyr1131) BioAssay ELISA Kit (IGF1Rb)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Insulin-Like Growth Factor I Receptor, beta, phosphorylated (Tyr1131) BioAssay ELISA Kit (IGF1Rb)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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