HSP104 is a molecular chaperone required for stress tolerance and for maintenance of [psi（+）] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 can protect yeast cells against high temperature and high concentration of ethanol but mutation studies have shown this protein is not required for normal growth. Hsp104 was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
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来源宿主
酿酒酵母中重组表达
溶解建议
Sterile filtered colorless solution. The HSP-104 protein solution contains 20mM Tris-HCl， pH 7.4，100mM NaCl. 2mM EDTA and 5% Glycerol.
保存建议
Store at 4℃ if entire vial will be used within 2-4 weeks.
Store， frozen at -20℃ for longer periods of time.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Avoid multiple freeze-thaw cycles.
