GroEL， HSP60 is a chaperonin located in the mitochondria which is responsible for the transportation & refolding of proteins from the cytoplasm directly into the mitochondrial matrix. GroEL is regulated by the HSP10 cochaperonin， which is a single heptameric protein ring having a molecular mass of 10 kDa which form a unique complex with HSP60. HSP10， GroES coordinates the ATPase activity of the HSP60 subunits in order to allow the release of bound polypeptide in a manner that is productive for its correct folding.
The GroEL protein having the NCBI accession number NP_002147 was purified by using conventional chromatography techniques.
序列
请咨询我们客服或者请查看品牌官方网站
来源宿主
大肠杆菌中重组表达
溶解建议
Sterile filtered colorless solution. The GroEL protein contains 25mM Tris-HCl buffer pH-7.5， 100mM NaCl， 5mM DTT and 10% Glycerol.
保存建议
Store at 4℃ if entire vial will be used within 2-4 weeks.
Store， frozen at -20℃ for longer periods of time.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Avoid multiple freeze-thaw cycles.
