- 中文名称
Histone H2A.X, phosphorylated, Human (H2A histone family member X, H2A.FX, H2A/X, H2AFX) BioAssay Flow Cytometry Kit
- 英文名字
- Histone H2A.X, phosphorylated, Human (H2A histone family member X, H2A.FX, H2A/X, H2AFX) BioAssay Flow Cytometry Kit
- 供应商
- USBiological
- 产品货号
- H5110-08Y
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Kit Components:H5110-08Y1: Histone H2A.X, phosphorylated (S139) Mab (FITC) 1x370ul Supplied as a liquid in PBS, 5mg/ml BSA, 0.05% sodium azide. Store at -20°C.H5110-08Y2: IgG, Normal mouse (FITC) 1x350ul of FITC-conjugated mouse antibody Supplied as a liquid in PBS, 5mg/ml BSA, 0.05% sodium azide. Store at -20°C.H5110-08Y3: Fixation Solution, 16X. 1x5ml Supplied as a liquid in 37% formaldehyde, 10-15% methanol. Store at room temperature.H5110-08Y4: Wash Solution, 10X. 1x2ml Supplied as a liquid in PBS, 1% saponin. Store at room temperature.H5110-08Y5: Supplied as a liquid: 100mM HEPES, pH 7.4, 1.4M sodium chloride, 25mM CaCl2, 5% saponin, 0.2um filter sterilized. Store at room temperature. Storage and Stability:Aliquot and store H5110-08Y1 and H5110-08Y2 at -20°C. Store other components at RT. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Kit DescriptionQuantity: Sufficient reagents for 100 assays per kit.Storage: Upon receipt, refer to individual components for storage conditions. Anti-phospho Histone H2A.X, FITC conjugate and Normal Mouse IgG, FITC conjugate may be stored at 4°C forup one month, however storage at -20°C is recommended. Aliquot FITC-conjugated antibodies to avoid repeated freeze-thaw cycles. All other components are stored at room temperature.Use: The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by the addition of the anti-phospho-Histone H2A.X, FITC conjugate. Cells are then scanned in a flow cytometer to quantitate the number of cells staining positive for phosphorylated Histone H2A.X.Please refer to the User Manual for further information and a detailed assay procedure.Technical Information for Kit ComponentsAnti-phospho-Histone H2A.X, FITC conjugate (mouse monoclonal IgG1)Product Description: Mouse monoclonal IgG1 specific for human histone H2A.X phosphorylated at serine residue 139, conjugated to fluorescein isothiocyanate (FITC).Immunogen: KLH-conjugated, synthetic peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. Broad species cross-reactivity is expected based on conservation of amino acid sequence.Normal Mouse IgG, FITC ConjugateMouse IgG purified by ion-exchange chromatography from pooled mouse serum, conjugated to fluorescein isothiocyanate (FITC).Quality Control TestingLog phase Jurkat cells were treated with staurosporine (1ug/ml) for the indicated time. Histone H2A.X (Ser139) phosphorylation was detected as described in the assay manual and the cells were analyzed on a Becton-Dickinson. See picture 1.
- 产品特点
- 针对Histone H2A.X, phosphorylated, Human (H2A histone family member X, H2A.FX, H2A/X, H2AFX) BioAssay Flow Cytometry Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Histone H2A.X, phosphorylated, Human (H2A histone family member X, H2A.FX, H2A/X, H2AFX) BioAssay Flow Cytometry Kit产品后将其置于RT/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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