- 中文名称
Histones Control Kit, BioAssay (+/-sodium butyrate)
- 英文名字
- Histones Control Kit, BioAssay (+/-sodium butyrate)
- 供应商
- USBiological
- 产品货号
- H5110-06B
- 产品报价
- ￥1/20x50ug
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders (1). In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3 (2). The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity (3).In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. The histones are a group of water and dilute acid soluble basic proteins found associated with DNA in chromosomes. They are characterized by relatively high levels of lysine and arginine. Although histones are classified into a limited number of types of fractions (see below) with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues. Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development. They are believed to play a role in gene activity and cellular metabolism.Histones are believed to be regularly arranged in the deep groove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.With the exception of H1, the primary structures of the calf thymus histones have been determined. Comparisons with the structures for histones from other sources indicate that the histones rank among the most highly conserved (low mutation rate) proteins in nature. Molecular Weights of Histones:Lysine Rich (H1, f1): ~ 21,500 Slightly Lysine Rich (H2a, f2a2): 14,004 Slightly Lysine Rich (H2b, f2b): 13,774 Arginine Rich (H3, f3): 15,324 Arginine Rich (H4, f2a1): 11,282Applications: Western Blot Analysis: 10ug of histones from untreated and sodium butyrate-treated HeLa cells were used as a positive control for immunoblot analysis using 2ug/ml anti-acetyl Histone H4, 1ug/ml anti-acetyl-Histone H3 and 1:3000 dilution of anti-hyperacetylated Histone H4 (Penta). 10ug Control Histones, untreated or sodium butyrate-treated, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2ug/ml anti-acetyl-Histone H4. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.Kit Components:H5110-06B1 Control Histones, untreated, (HeLa cell acid extract), 10x50ug of precipitated core histones, lyophilized from sterile water. H5110-06B2 Control Histones, sodium butyrate-treated, (HeLa cell acid extract), 10x50ug of precipitated core histones, lyophilized from sterile water.Storage and Stability: Stable for 2 years at -20°C from date of shipment, when stored with provided desiccant. Stable for 6 months at -70°C when reconstituted. To rehydrate, aseptically reconstitute to 1ug/ml with sterile water. Aliquot to avoid repeated freezing and thawing.
- 产品特点
- 针对Histones Control Kit, BioAssay (+/-sodium butyrate)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Histones Control Kit, BioAssay (+/-sodium butyrate)产品后将其置于-20°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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