- 中文名称
Histone Deacetylase (HDAC), Fluorogenic Assay Kit, BioAssay
- 英文名字
- Histone Deacetylase (HDAC), Fluorogenic Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- H5109-67
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The HDAC Fluorescent Activity Assay kit is a complete assay system designed to measure histone deacetylase (HDAC) activity in cell extracts, nuclear extracts, immunoprecipitates, and purified enzymes. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC2 and a potent HDAC inhibitor, Trichostatin A, for the positive and negative control. The HDAC Fluorescent Activity Assay is based on the unique fluorogenic substrate and Developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, comprised of an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g. HeLa nuclear extract or purified HDAC enzymes). The deacetylation will sensitize the substrate, causing further treatment with the Lysine Developer to produce a fluorophore that can then be measured using a fluorescence reader. Components: H5109-67A: HDAC2 Recombinant Human2x1ugH5109-67B: HDAC Fluorogenic Substrate1x50ulH5109-67C: HDAC Developer 2x6ml(contains Trichostatin A)H5109-67D: Trichostatin A1x100ulH5109-67E: HDAC Assay Buffer1x10mlH5109-67F: Microtiter plate1x96 wellsApplications: Suitable for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Storage and Stability:Aliquot to avoid repeated freezing and thawing and store at recommended temperature. Store H5109-67A, H5109-67B and H5109-67C at -70°C. Store H5109-67D and H5109-67E at -20°C. Store H5109-67F at room temperature. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
- 产品特点
- 针对Histone Deacetylase (HDAC), Fluorogenic Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Histone Deacetylase (HDAC), Fluorogenic Assay Kit, BioAssay产品后将其置于-70°C/-20°C/RT保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
