- 中文名称
Histone Deacetylase class 2a, Fluorogenic BioAssay Kit
- 英文名字
- Histone Deacetylase class 2a, Fluorogenic BioAssay Kit
- 供应商
- USBiological
- 产品货号
- H5109-66
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Fluorogenic HDAC Class2a Assay Kit is a complete assay system designed to measure histone deacetylase (HDAC) class2a activity in cell extracts, nuclear extracts, immunoprecipitates, and purified enzymes. It comes in a convenient 96 format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC4 and a potent HDAC inhibitor, Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC Class 2a Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC class2a activity level. First, the HDAC fluorometric substrate is incubated with a sample containing HDAC activity (e.g. HeLa nuclear extract or purified HDAC4 enzyme). The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader.Kit Components: 1. HDAC4 human recombinant enzyme: 1x0.1ug. Store at -70°C.2. Fluorogenic HDAC substrate (5mM): 1x50ul. Store at -70°C.3. 2x HDAC Developer (contains Trichostatin A) (50uM): 1x6ml. Store at -70°C.4. Trichostatin A (200uM): 1x100ul. Store at -20°C.5. Histone Deacetylase, Assay Buffer (HDAC): 1x10ml. Store at -20°C.6. Black, low binding NUNC black microtiter plate: 1 plate. Store at room temperature.APPLICATIONS: Suitable for studying enzyme kinetics and screening small molecular inhibitorsof HDAC class2a for drug discovery and HTS applications. Other applications not tested.Storage and Stability:Store buffer and standard at -20°C. Store enzyme and substrate at -70°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Histone Deacetylase class 2a, Fluorogenic BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Histone Deacetylase class 2a, Fluorogenic BioAssay Kit产品后将其置于RT/-20°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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