- 中文名称
Histone Deacetylase Assay Kit (Histone H4 Peptide Substrate), BioAssay
- 英文名字
- Histone Deacetylase Assay Kit (Histone H4 Peptide Substrate), BioAssay
- 供应商
- USBiological
- 产品货号
- H5109-60
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAc (histone deacetylase) activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAc inhibitor sodium butyrate is provided to demonstrate specificity of the deacetylase activity.Quantity: Approximately 100 assays per 100ug of labeled peptide (the exact amount will vary according to labeling efficiency). Use of 5000-10,000 CPM of [3H]-acetyl histone H4 for each assay is recommended.Quality Control Testing and Research Applications:[3H]-acetylation of Histone H4 peptide: 70% of [3H]-acetate was incorporated into 100ug Histone H4 peptideHDAc Assay:Experiment 1: Dependence of [3H]-acetate release (HDAc Activity) on the amount of HeLa Nuclear Extract. Increasing amounts of HeLa nuclear extract were incubated at room temperature with [3H]-acetyl-Histone H4 peptide (10,000CPM). Released [3H]-acetate was measured using a scintillation counter. HeLa nuclear extract is a source of HDAc activity.Experiment 2: Inhibition of [3H]-acetate release (HDAc Activity) by Sodium Butyrate [3H]-acetyl-Histone H4 peptide was incubated overnight at room temperature with 20ug HeLa nuclear extract in the presence or absence of Sodium Butyrate. 250mM Sodium Butyrate inhibited release of [3H]-acetate by ~96%.Storage and Stability: After receipt, store individual components at temperatures recommended above and on component labels. This kit is stable for 6 months from date of shipment.
- 产品特点
- 针对Histone Deacetylase Assay Kit (Histone H4 Peptide Substrate), BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Histone Deacetylase Assay Kit (Histone H4 Peptide Substrate), BioAssay产品后将其置于RT/4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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