- 中文名称
Hepatocyte Isolation System (Tissue Dissociation/Cell Isolation), BioAssay Kit
- 英文名字
- Hepatocyte Isolation System (Tissue Dissociation/Cell Isolation), BioAssay Kit
- 供应商
- USBiological
- 产品货号
- H2006-02
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Most traditional methods published for isolating hepatocytes use crude and partially purified enzyme preparations including various types of collagenase and other proteases. More recently the use of better characterized preparations of collagenase such as Types 1 and 4 have provided better results. All crude collagenase preparations can contain lot-variable contaminating proteases, esterases and other enzymes requiring researchers to pre-screen several lots of enzyme and/or continually modify isolation parameters and protocols.The Hepatocyte Isolation System has been developed to provide researchers with a reliable, convenient, and consistent hepatocyte cell isolation system. By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes. In addition, each lot is tested by isolating hepatocytes from adult rat to assure performance, reliability, and consistent yield of viable cells.The method is based on that described by Berry, M.N., modified by Seglen, P.O. (Methods in Cell Biology, vol XIII, David M. Prescott ed., Academic Press, 1976; Chapter 4, Preparation of Isolated Rat Liver Cells, pp 29-83), and further optimized in conjunction with several researchers.The Kit contains sufficient materials for five separate adult rat liver perfusions or 5-10 adult mouse perfusions. For larger or smaller tissue applications, prepare proportionate volumes of reagents at each step and combine them in the same ratio as described in the protocol.Kit Components:Enzyme Vials, Collagenase, ElastaseDNase Vials10X CMF-Hank’s HBSS Balanced Salt SolutionL-15 Media Powder , 1x1L0.15M MOPS Buffer MOPS, 1x75ml7.5% Sodium Bicarb. NAH, 1x100ml
- 产品特点
- 针对Hepatocyte Isolation System (Tissue Dissociation/Cell Isolation), BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Hepatocyte Isolation System (Tissue Dissociation/Cell Isolation), BioAssay Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
