- 中文名称
Hepatitis C Virus Protease, Fluorometric 490, BioAssay Kit (HCV)
- 英文名字
- Hepatitis C Virus Protease, Fluorometric 490, BioAssay Kit (HCV)
- 供应商
- USBiological
- 产品货号
- H1924-82
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Kit Size: 200 Assays (96-well) or 500 assays (384-well)This kit is optimized to detect the activity of Hepatitis C Virus NS3/4A protease using anEDANS/DABCYL FRET peptide substrate, with its fluorescence monitored at Ex/Em=340 nm/490nm upon proteolytic cleavage. It provides ample materials to perform 200 assays in a 96-well format and 500 assays in a 384-well plate. Convenient Format: Complete kit including all the assay components.Optimized Performance: Optimal conditions for the detection of HCV NS3/4A protease activity.Enhanced Value: Less expensive than the sum of individual components.High Speed: Minimal hands-on time.The NS3/4A protease of Hepatitis C Virus (HCV) is required for the cleavage of viralnonstructural polyprotein at the NS3-NS4A, NS4A-NS4B, NS4B-NS5A, and NS5A-NS5B sites. These cleavages are essential for the maturation of the viral proteins. Thus, this protease has become one of the key targets for developing anti-HCV drugs.The 490 HCV Protease Assay Kit provides a convenient assay for high throughputscreening of HCV NS3/4A protease inhibitors and for continuous quantification of HCV NS3/4A protease activity using fluorescence resonance energy transfer (FRET) peptide. The sequence of this FRET peptide is derived from the cleavage site of NS4A/NS4B. The cysteine on the natural cleavage site is replaced with aminobutyric acid (Abu) and the scissile amide bond with an ester bond. These modifications improved kcat/Km values by more than 100 fold and enabled the detection of the activity of NS3/4A protease at subnanomolar concentrations. In the FRET peptide, the fluorescence of EDANS is quenched by DABCYL. Upon cleavage into two separate fragments by HCV NS3/4A protease at the Abu-Ala bond (see Scheme 1), the fluorescence of EDANS is recovered, and can be monitored at excitation/emission = 340 nm/490 nm.Kit Components:Components C and D can be stored at room temperature for convenience.Component A: Microtiter Plate 1x200 wells HCV NS3/4A protease substrate (250ul)EDANS/DABCYL FRET peptide, Ex/Em=340nm/490 nm upon cleavageComponent B: EDANS, fluorescence reference standard (100uM DMSO solution, 10ul)Ex/Em=340nm/490nmComponent C: 2X Assay buffer (25ml)Component D: Stop solution (15ml)Component E: DTT (1 M, 1ml)Component F: Pep4AK (150uL, 600uM), HCV NS3 protease cofactorMaterials Required (but not provided):96-well or 384-well microplate: Black microplate can provide better signal to noise ratio.Fluorescence microplate reader: Capable of detecting emission at 490±30nm with excitation at 340±3 nm.HCV NS3/4A protease: HCV NS3/4A protease can be produced from E. coli1-3.Storage and Handling:Store all kit components at -20°C, and keep Components A and B away from light. If used frequently.
- 产品特点
- 针对Hepatitis C Virus Protease, Fluorometric 490, BioAssay Kit (HCV)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Hepatitis C Virus Protease, Fluorometric 490, BioAssay Kit (HCV)产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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