- 中文名称
Hepatitis B Virus E Antigen (HBeAg), ELISA Kit, BioAssay (Human)
- 英文名字
- Hepatitis B Virus E Antigen (HBeAg), ELISA Kit, BioAssay (Human)
- 供应商
- USBiological
- 产品货号
- H1910-40K
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Hepatitis B Virus E Antigen (HBeAg) ELISA Kit is a qualitative in vitro enzyme immunoassay for the detection of HBeAg in human serum or plasma.Principle of the Assay:Purified Anti-HBe is coated on the solid phase of multi-wells. Serum samples and Anti-HBe-HRP conjugate are added to coated wells. After incubation, if HBeAg is present in the sample, a complex of Anti-HBe-HBeAg-Anti-HBe-HRP will form. Wells are washed to remove other unbound serum components and incubated with substrate (TMB) to form a colored product. The absorbance at 450nm is measured to indicate the presence or absence of HBeAg in the sample. The test is sensitive, reproducible and easy to operate.Kit Components:H1910-40K1: Microtiter Plate, 1x96 wells. Coated with Anti-HBeH1910-40K2: Enzyme Conjugate, 1x6.5mlH1910-40K3: Positive Control Serum, 1x1mlH1910-40K4: Negative Control Serum, 1x1mlH1910-40K5: Wash Buffer, 20X, 1x50ml. Dilute 1:20 prior to use.H1910-40K6: Substrate A, 1x8.5mlH1910-40K7: Substrate B, 1x8.5mlH1910-40K8: Stop Solution, 1x8.5mlStorage And Stability:Store all kit components at 4°C. Kit is stable for 6 months after receipt.Test Procedure:1. Set one blank, two positive controls and two negative controls for each assay. Add 50ul serum sample, positive control serum and negative control serum into the coated wells. 2. Add one drop (approximately 50ul) of Enzyme Conjugate into the wells, except the blank wells. Mix thoroughly, cover the wells with seal paper, and incubate for 30-60 minutes at 37°C.3. Wash the wells. Manual Wash Procedure: Remove the liquid completely from the coated wells. Fill the wells with wash solution and then remove the liquid completely. Repeat 5 times.Automatic Wash Procedure: Select the automatic operation of washing to 5 times; ensure liquid has been removed completely after the last wash.4. Add one drop (approximately 50ul) of Substrate A and B respectively to each well, seal the plate with seal paper, and incubate for 10-15 minutes at 37°C. Avoid exposure to light.5. Add one drop (approximately 50ul) of Stop solution into each well and then mix thoroughly to terminate the reaction. Measure the absorbance at 450nm against the blank or measure the absorbance at 450nm/630-690nm.Interpretation of Results:Cut-off Value (COV) Calculation:COV = average OD450 of negative controls x 2.1Positive: OD450 of sample ≥ COVNegative: OD450 of sample < COVInvalid: If the average OD of negative controls is ≥ 0.1, the result is invalid. Repeat the test. If the problem persists, contact the local distributor.Note: If the absorbance of negative controls is below 0.05, calculate it as 0.05. If the absorbance of negative controls is above 0.05, calculate it as its original value.Performance Characteristics:Sensitivity: 2NCU/ml (per Biological Reference Reagents of Chinese Clinical Test Center), OD ≥ 1.500Specificity: The average OD of 20 normal negative samples is ≤ 0.030Precision: %CV ≤ 15% (n= 10)
- 产品特点
- 针对Hepatitis B Virus E Antigen (HBeAg), ELISA Kit, BioAssay (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Hepatitis B Virus E Antigen (HBeAg), ELISA Kit, BioAssay (Human)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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