Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (<1uM ≈ 650ng/ml) exerting regulatory functions such as repression of nonspecific delta-aminolevulinate synthase expression and induction of microsomal hemin oxygenase-1. Hemin can stimulate growth of oral bacteria involved in gingivitis and is an indicator of possible pathological conditions when found in the urine or feces. The Hemin Assay Kit utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (lambda = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160pg (10-250fmol) range.Kit Components:Hemin Assay Buffer 1x25mlHemin Probe 1x200ulEnzyme Mix 1x1vialHemin Substrate 1x1mlHemin Standard (1nmol) lyophilized,1x1vialStorage and Stability:Store the kit at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
