- 中文名称
Heme Assay Kit, BioAssay
- 英文名字
- Heme Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- H1847-64
- 产品报价
- ￥1/250Tests
- 产品说明书
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- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Heme is one important member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm, and is a key prosthetic group for various essential proteins such as hemoglobin, cytochromes, catalases and peroxidases. Heme determination is widely practiced by researchers of various blood diseases.Simple, direct and automation-ready procedures for measuring heme concentration are becoming popular in Research and Drug Discovery. Heme Assay Kit is based on an improved aqueous alkaline solution method, in which the heme is converted into a uniform colored form. The intensity of color, measured at 400 nm, is directly proportional to the heme concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.Applications:Direct Assays: total heme in blood, serum, plasma, urine, heme-carrying enzymes. Pharmacology: effects of drugs on heme metabolism.Drug Discovery: HTS for drugs that modulate heme levels.Key Features:Sensitive and accurate. Linear detection range 0.6–125uM heme in 96-well plate assay.Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Safety. Reagents are non-toxic.Versatility. Assays can be executed in 96-well plate or cuvet.Kit Contents: (250 tests in 96-well plates)Reagent: 50mlCalibrator: 10ml (equivalent to 62.5uM heme)Storage conditions. The kit is shipped room temperature. Store reagent and Calibrator at 4°C. Shelf life: 12 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Procedure using 96-well plate:1. Blank and Calibrator. Pipette 50ul water (Blank) and 50ul Calibrator into wells of a clear bottom 96-well plate. Transfer 200ul water into the blank and Calibrator wells. The diluted calibrator is equivalent to 62.5uM heme.2. Samples. Serum and plasma samples can be assayed directly (n=1). Blood samples should be diluted 100-fold in distilled water (n=100). Transfer 50ul samples into wells (important: avoid bubble formation during the pipetting steps). Add 200ul Reagent to sample wells and tap plate lightly to mix.3. Incubate 5 min at room temperature. Read OD at 380-420nm (peak 400nm).Procedure using cuvette:1. Transfer 100ul sample and 1000ul Reagent into a cuvet and tap lightly to mix. Read OD at 380-420nm (peak 400 nm) against water.2. Transfer 100ul Calibrator and 1000ul water to cuvet. Read OD at 400nm against water.Calculation:Subtract blank OD (water) from the Calibrator and Sample OD values. The total heme concentration of Sample is calculated as=ODSAMPLE–ODBLANKODCALIBRATOR–ODBLANKX 62.5 x n (uM)ODSAMPLE, ODCALIBRATOR and ODBLANK are OD values of the sample,the Calibrator and water. n is the dilution factor (100 for bloodsamples).Conversions: 1mg/dL heme equals 15.3uM, 0.001% or 10 ppm.Materials Required, But Not Provided:Pipeting devices and accessories. Procedure using 96-well plate: Clear-bottom 96-well plates (e.g. Corning Costar) and plate reader. Procedure using cuvette: Cuvets and spectrophotometer.Examples:Heme was determined using the 96-well plate protocol. The values were 27.3± 0.2uM for rat serum, 7.8 ± 0.4uM for human plasma and 11.2 ± 0.2mM for a mouse whole blood sample.
- 产品特点
- 针对Heme Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Heme Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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