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中文名称

Helicobacter pylori IgM, BioAssay ELISA Kit, Qualitative

英文名字
Helicobacter pylori IgM, BioAssay ELISA Kit, Qualitative
供应商
USBiological
产品货号
H1840-24J
产品报价
¥1/1Kit
产品说明书
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背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Sample Type:SerumIntended Use:The Helicobacter pylori IgM Test Kit is intended for use in evaluating the serologic status to H. pylori.Introduction:Helicobacter pylori is a spiral bacterium cultured from human gastric mucosa discovered by B.J. Marshall in 1982. Studies have indicated that the presence of H. pylori is associated with a variety of gastrointestinal diseases including gastritis, duodenal and gastric ulcers, non-ulcer dyspepsia, and gastric adenocarcinoma and lymphoma. The organism is present in 95-98% of patients with duodenal ulcers and 60-90% of patients with gastric ulcers. All of the testing performed on biopsy samples is subject to errors related to sampling and interference of contaminated bacteria. An ELISA test for the presence of H. pylori specific IgM antibody is the technique of choice for serologic tests because of its accuracy and simplicity.Test Principle:Purified H. pylori antigen is coated on the surface of microwells. Diluted serum is added to the wells, and the H. pylori IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away. Enzyme conjugate is added, which binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and a solution of TMB Reagent is added. The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of IgM-specific antibody in the sample. The results are read by a microwell reader compared in a parallel manner with calibrator and controls.Kit Components:Antigen-Coated Wells (1 plate, 96 wells), Microtiter Wells coated with purified H. pylori antigenEnzyme Conjugate Reagent (13ml), Contains goat anti-human IgM conjugated to horseradish peroxidase with preservativesSample Diluent (22ml), Contains phosphate buffer with preservativesNegative Control (150ul/vial), Contains H. pylori IgM negative serum with preservativesCut-off Calibrator (150ul/vial), Contains H. pylori IgM weak positive serum with preservatives; H. pylori IgM Index = 1Positive Control (150ul/vial), Contains H. pylori IgM positive serum with preservativesWash Buffer Concentrate (20X) (50ml), Contains phosphate buffer and Tween 20TMB Reagent (11ml), Contains 3, 3’, 5, 5’ tetramethylbenzidine (TMB) stabilized in buffer solutionStop Solution -1N HCl (11ml), Diluted hydrochloric acidStorage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对Helicobacter pylori IgM, BioAssay ELISA Kit, Qualitative该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Helicobacter pylori IgM, BioAssay ELISA Kit, Qualitative产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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