- 中文名称
Heat Shock Protein 70B’ (HSP70B') BioAssay ELISA Kit
- 英文名字
- Heat Shock Protein 70B’ (HSP70B') BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- H1834-39
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Hsp70 system is a highly conserved family of ubiquitous proteins found in all prokaryotes and in cellular compartments of eukaryotic organisms (1). Multiple members of this family are involved in protein folding and several other cellular functions (2). Some forms are constitutively expressed in cells, while others are only inducible by metabolic stress (2). Various stressors that induce Hsp70 include heat shock, hypoxia, UV irradiation, CdCl2 and arsenite (1,3). Basal levels of constitutive Hsp70 are found in major intracellular compartments and the inducible Hsp70s are predominantly cytoplasmic and nuclear in distribution (2). There are at least 11 genes for proteins of the human Hsp70 family, which code for a group of highly related proteins ranging from 66 to 78kD (2). The multiple members of this family vary with their basal expression levels and inducibility in response to different stressors (2). The Hsp70 chaperones have two major functional domains (4). The highly conserved NH2-terminal domain has ATPase activity and binds to ADP and ATP very tightly, and COOH-terminal binds to polypeptides (4). Hsp70 is known to bind preferentially to unfolded and partially folded proteins and, prevent their aggregation or misfolding (2). The nomenclature of the different members of Hsp70 family is extensive and is based on cellular distribution and inducibility (2). Human Hsp70B’ is a variant Hsp70 that is more basic than the major Hsp70 and has different stress-induction characteristics (5,6). There have been several reports of Hsp70 variants, but most of them are products of different postranslational modifications (5). Hsp70B’ is a product of a separate gene which is devoid of introns similar to Hsp70 (5). The Hsp70B’ gene has 77% sequence identity to Hsp70 gene and 70% identity to Hsc70 cDNA with highest sequence divergence at the 3’- end (5). Promoter studies have shown Hsp70B’ to be a unique member of the Hsp70 family (5). Unlike Hsp70 which shows basal levels of expression and is induced by heat and various stressors, Hsp70B’ is strictly heat-inducible and shows no basal levels (5). One study reported the use of hsp70B’ as a target gene for studying single nucleotide polymorphisms (SNP) in biopsy samples of human prostate cancer patients (7). However, the use of Hsp70B’ protein as a common biomarker of stress in heat shock protein research is still at its infancy.Number of Samples: 1 standard curve, 39 samples in duplicate or 2 standard curves and 30 samples in duplicate Sensitivity: 60pg/ml Range: 0.156-10ng/mlSample Volume: 100ul/well Incubation Time: <4.5 hours Wavelength Setting: 450nm Materials Included:• Anti-Hsp70B′ Immunoassay Plate, • 5X Hsp70B, Extraction Reagent, • Recombinant Hsp70B′ Standard, • Sample Diluent,• 10X Wash Buffer, • Anti-Hsp70B′, • Anti-Hsp70B′ Diluent, • Anti-Rabbit IgG: HRP Conjugate,• Anti-Rabbit IgG: HRP Conjugate Diluent • TMB Substrate, • Acid Stop Solution, • Instruction Manual Storage and stability:All reagents are stable as supplied at 4°C, except the Recombinant Hsp70B′ Standard, which should be stored at -20°C. For optimum storage, the Recombinant Hsp70B′ Standard should be aliquotted into smaller portions and stored at -20°C. Avoid repeated freeze/thaw cycles.
- 产品特点
- 针对Heat Shock Protein 70B’ (HSP70B') BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Heat Shock Protein 70B’ (HSP70B') BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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