- 中文名称
Heat Shock Protein 27 (HSP27), Total, BioAssay Sandwich ELISA Kit (Qualitative)
- 英文名字
- Heat Shock Protein 27 (HSP27), Total, BioAssay Sandwich ELISA Kit (Qualitative)
- 供应商
- USBiological
- 产品货号
- H1830-55A
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Total HSP27 Sandwich ELISA kit is a qualitative, solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total HSP27 protein. An HSP27 antibody has been coated onto the microwells. After incubation with cell lysates, both nonphospho- and phospho-HSP27 are captured by the coated antibody. Following extensive washing, an HSP27 mouse monoclonal antibody is added to detect the captured HSP27 protein. HRP-linked anti-mouse antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of total HSP27 protein. Antibodies in this kit are custom formulations specific to the kit.Heat shock protein (HSP) 27 is one of the small Hsps that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, 78 and 82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6)Specificity/Senstivity: This ELISA kit that detects endogenous levels of total HSP27 protein. Using Phospho-HSP27(Ser78) Sandwich ELISA Kit, a significant induction of phospho-HSP27 (Ser78) in HeLa cells treated with UV light can be detected. However, the level of total HSP27 (phospho and non-phospho), detected by this kit remains unchanged see (Figure 1). COS cells treated with UV light show similar results. Species Crossreactivity: Human and monkeyKit Components:H1830-55A1: Microtiter Plate, 1x96 wells (12 strips of 8 wells). Coated with HSP27 antibodyH1830-55A2: HSP27 Detection Antibody, 1x11ml. Green solution.H1830-55A3: IgG (HRP), 1x11ml. Anti-mouse IgG linked to HRP. Red solution.H1830-55A4: TMB Substrate, 1x11ml. Colorless solution.H1830-55A5: Stop Solution, 1x11ml. Colorless solutionH1830-55A6: Wash Buffer, 20X, 1x25ml. Colorless solution.H1830-55A7: Sample Diluent, 1x25ml. Blue solution.H1830-55A8: Cell Lysis Buffer, 10X, 1x15ml. Yellowish solution.Storage and Stability:Store H1830-55A8 Cell Lysis Buffer, 10X at -20°C Store other components at 4°C. Kit is stable for up to 6 months.
- 产品特点
- 针对Heat Shock Protein 27 (HSP27), Total, BioAssay Sandwich ELISA Kit (Qualitative)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Heat Shock Protein 27 (HSP27), Total, BioAssay Sandwich ELISA Kit (Qualitative)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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