- 中文名称
Haptoglobin, Human BioAssay ELISA Kit (HP)
- 英文名字
- Haptoglobin, Human BioAssay ELISA Kit (HP)
- 供应商
- USBiological
- 产品货号
- H1820-21
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Haptoglobin (abbreviated as Hp) is a protein in the blood plasma that binds free hemoglobin released from erythrocytes with high affinity and removes it from the circulation to prevent kidney injury, and iron loss following hemolysis. Also, haptoglobin prevents bacteria from using the iron present in hemoglobin to grow, regulates the activity of many cell types of the immune system, and is an extracellular chaperone. Haptoglobin, as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. In addition, haptoglobin acts as a potent immunosuppressor of lymphocyte function and modulates the helper T-cell type 1 and type 2 (Th1/Th2) balance within the body. Haptoglobin plays a role in stimulation of angiogenesis and has highly potent cholesterol crystallization-promoting activity, also. Changes in the measured concentrations of haptoglobin in serum may help to assess the disease status of patients with inflammations, infections, malignancy etc. (increases) as well as in haemolytic conditions (decreases). Haptoglobin in its simplest form consists of two a- and two b-chains, connected by disulfide bridges. The chains originate from a common precursor protein which is proteolytically cleaved during protein synthesis. Haptoglobin exists in two allelic forms in the human population, so called Hp1 and Hp2; the latter one having arisen due to the partial duplication of Hp1 gene. Three phenotypes of haptoglobin therefore are found in humans: Hp1-1, Hp2-1, and Hp2-2. Hp of different phenotypes have been shown to bind hemoglobin with different affinities. Hp 1-1 is biologically the most effective in binding free hemoglobin and suppressing inflammatory responses associated with free hemoglobin. Hp 2-2 is biologically the least active, and Hp 2-1 is moderately active. The possible association of allelic polymorphism of haptoglobin with various pathologic conditions such as cardiovascular disease, autoimmune disorders, malignancy has been studied. Hp 2-2 phenotype presents characteristics of being an independent risk factor for cardiovascular disease (CVD) and more significant in diabetic CVD. In chronic kidney disease (CKD), due to various processes that take place simultaneously, the combined effect is different in the elderly and the young. The functional differences between the Haptoglobin phenotypes are most probably due to their antioxidative property and to macrophage activation with differential release of cytokines.Intended Use:Human Haptoglobin ELISA kit is to be used for the in vitro quantitative determination of human Haptoglobin in human serum, human plasma, cell lysate and buffered solution. The assay will recognize both native and recombinant human Haptoglobin. This kit has been configured for research use only and is not to be used in diagnostic procedures.Sensitivity:The minimal detectable dose of human Haptoglobin was calculated to be 0.78ng/ml, by subtracting two standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.Specificity:The following substances have been tested and found to have no cross-reactivity: human Albumin, IgG, Transferrin, Fibrinogen, a-2 marcroglobulin, Complement C3, Antitrypsin.Test Principle:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human Haptoglobin. Samples are pippetted into these wells. Nonbound Haptoglobin and other components of the sample should be removed by washing, then biotin- conjugated monoclonal antibody specific to Haptoglobin added. In order to quantitatively determine the amount of Haptoglobin present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured Haptoglobin.Kit Components:Microplate: 1x96 wellsIncubation buffer: 1x30mlWashing buffer, (10x): 1x100mlStandard protein: 1 glass vial lyophilizedStandard/sample dilution buffer: 1x25mlSecond antibody: 1 glass vial lyophilizedAV-HRP, (100x): 1x150ulSecondary antibody/AV-HRP dilution buffer: 1x25mlSubstrate (TMB): 1x20mlStop solution: 1x20mlStorage and Stability:Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Haptoglobin, Human BioAssay ELISA Kit (HP)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Haptoglobin, Human BioAssay ELISA Kit (HP)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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