- 中文名称
Haptoglobin, Human (HP, BP, HP2-ALPHA-2, HPA1S, MGC111141) BioAssay ELISA Kit
- 英文名字
- Haptoglobin, Human (HP, BP, HP2-ALPHA-2, HPA1S, MGC111141) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- H1820-04J
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Acute phase proteins are plasma proteins which increase in concentration following infection,inflammation or trauma. The first acute phase proein to be recognized was discovered in humans by Tillet and Frances in 1930 (1). Haptoglobin (Hp) is a heterogeneous plasma protein mostly synthesized by the liver. The haptoglobin monomer consist of two heavy chains, beta chains (40kD) and two light chains, alpha chains, alpha 1 (9kD) and alpha 2 (16kD) that are linked disulfide bonds. The three major haptoglobin types are; Hp1-1 which is monomeric (98kD), Hp1-2 is polymeric at about 200kD, and Hp2-2 at about 400kD. The levels in serum rise quickly following acute tissue damage within 24 to 48 hours and also fall very rapidly once the stimulus is removed. In fact, Hp level are decreased in hemolytic anemia. Hp has a high affinity for hemoglobin (Hb) and its function appears to be to prevent loss of Hb in urine which would lead to loss of iron. Investigations over the past few years have shown that quantification of Hp in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in humans, but in companion animals and farm herds as well (2).Sample Type:Serum/PlasmaIntended Use:The Haptoglobin test kits are a highly sensitive twosite enzyme linked immunoassay (ELISA) formeasuring Haptoglobin in serum or plasma of Human. Crossreactivity with Cynomolgus Monkeys has been observed.Test Principle:The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay theHaptoglobin present in samples reacts with the anti-Haptoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Hp antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Hp. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Hp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Hp in the test sample. The quantity of Hp in the test sample can be interpolated from the standard curve constructed from the standards, andcorrected for sample dilution.Kit Components:Diluent concentrate (Running Buffer), One bottle containing 1x50ml of a 5X concentrated diluent running buffer.Wash solution concentrate, One bottle containing 1x50ml of a 20X concentrated wash solution.Enzyme-antibody conjugate 100X, One vial containing 1x150ul of affinity purified anti-Human Haptoglobin antibody conjugated with horseradish peroxidase in a stabilizing buffer.Chromogen-substrate solution, One vial containing 1x12ml of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.Stop solution, One vial containing 1x12ml 0.3M sulfuric acid.Anti-human haptoglobulin ELISA micro plate, Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Human Hp.Human haptoglobulin calibrator, One vial containing a lyophilized Human Haptoglobin calibrator.Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Haptoglobin, Human (HP, BP, HP2-ALPHA-2, HPA1S, MGC111141) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Haptoglobin, Human (HP, BP, HP2-ALPHA-2, HPA1S, MGC111141) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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