- 中文名称
Growth Hormone, Human (hGH, IGHD1B, Pituitary Growth Hormone, Somatotropin) BioAssay ELISA Kit
- 英文名字
- Growth Hormone, Human (hGH, IGHD1B, Pituitary Growth Hormone, Somatotropin) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- G9000-05H
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Human growth hormone (HGH, somatotropin) is a polypeptide secreted by the anterior pituitary. It has 191aa in length and has a molecular mass of approximately 22,000D. Its metabolic effects are primarily anabolic. HGH promotes protein conservation and is engaged in a wide range of mechanisms for protein synthesis. It also enhances glucose transport and facilitates glycogen storage. Its cascade of growth-promoting action is mediated by another family of peptide hormones, the somatomedins. HGH measurement is primarily of interest in the diagnosis and treatment of various forms of abnormal growth hormone secretion. Disorders caused by hyposecretion include dwarfism and unattained growth potential, and hypersecretion is associated with gigantism and acromegaly. Caution must be exercised in the clinical interpretation of growth hormone levels. These vary throughout the day, making it difficult to define a normal range or to judge an individual’s status based on a single determination. Many factors are known to influence the rate of growth hormone secretion, including periods of sleep and wakefulness, exercise, stress, hypoglycemia, estrogens, corticosteroids and L-dopa. Because of its similarity to prolactin and placental lactogen, earlier growth hormone immunoassays were often plagued with falsely high values in pregnant and lactating women. Because not all acromegalic individuals have elevated baseline levels of growth hormone, suppression tests based on glucose loading are of value in this context. In spite of the induced hyperglycemia, there is rarely a decrease from baseline levels in acromegaly. Growth hormone-deficient individuals have fasting and resting levels similar to those found in normal individuals. Various challenge tests have therefore been devised to differentiate them. For example, with the onset of deep sleep or after 15 to 20 minutes of vigorous exercise, growth hormone levels normally rise. Other tests of growth hormone responsiveness are based on the administration of L-dopa, arginine and insulin. Propanolol or estrogen are sometimes given in conjunction with the primary stimulus to accentuate the response. A small number of dwarfism cases have been documented in which both the basal level of HGH and the responses to challenge testing were normal. Such cases may involve tissue insensitivity to either growth hormone or the somatomedins, or immunoreactive but biologically inactive growth hormone. The Human Growth Hormone Enzyme Immunoassay provides a rapid, sensitive and reliable test. There is no cross-reactivity with HCG, TSH, LH, FSH and prolactin.Sample Type:SerumIntended Use:For the quantitative determination of the Human Growth Hormone (HGH) concentration in human serum.Test Principle:The HGH Quantitative Test Kit is based on the principle of a solid phase enzyme-linked immunosorbent assay (ELISA). The assay system utilizes a sheep anti-HGH antibody for solid phase (microtiter wells) immobilization and a mouse monoclonal anti-HGH antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in HGH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 45-minute incubation at room temperature, the wells are washed with water to remove unbound-labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution, and the color is changed to yellow and measured spectrophotometrically at 450nm. The concentration of HGH is directly proportional to the color intensity of the test sample.Kit Components:Sheep Anti-HGH-coated microtiter plate with 96 wells.Standard, 0ng/ml, 1x1vial Standard, 2.5ng/ml, 1x1vialStandard, 5ng/ml, 1x1vialStandard, 10ng/ml, 1x1vialStandard, 25ng/ml, 1x1vialStandard, 50ng/ml, 1x1vial Enzyme Conjugate Reagent, 1x13ml.TMB Reagent (One-Step), 1x11ml.Stop Solution (1N HCl), 1x11ml.Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Growth Hormone, Human (hGH, IGHD1B, Pituitary Growth Hormone, Somatotropin) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Growth Hormone, Human (hGH, IGHD1B, Pituitary Growth Hormone, Somatotropin) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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