- 中文名称
Glycosyltransferase Activity, BioAssay Kit
- 英文名字
- Glycosyltransferase Activity, BioAssay Kit
- 供应商
- USBiological
- 产品货号
- G8183-01
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Test Principle:The Glycosyltransferase Activity Kit provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all glycosyltransferases that use diphosphonucleotide sugars as donor substrates. This kit takes advantage of nucleotidase ENTPD3/CD39L3 as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide diphosphate, such as UDP or GDP, of glycosyltransferase reactions (Figure 1). The released inorganic phosphate is then detected by Malachite Green phosphate detecting reagents. The amount of inorganic phosphate released by the coupling phosphatase is equal to the nucleotide sugar consumed or glycoconjugate product generated; therefore, the rate of inorganic phosphate production reflects the kinetics of a glycosyltransferase reaction.Kit Components:1. *Coupling Phosphatase 1 (100ng/ml) NS0-expressed recombinant human ENTPD3/CD39L3 in 20mM Tris, 120mM NaCl, 4mM CaCl2, 20% glycerol, pH 7.5, 1x200ml 2. Phosphatase Buffer 1 (10X) 250mM Tris, 100mM CaCl2, pH 7.5, 1x1.5ml 3. *UDP (5mM) in 10mM sodium borate, pH 9.0, 1x100ml 4. MnCl2 (100mM), 2x1.5ml 5. Phosphate Standard (1mM KH2PO4), 1x 200ml 6. Malachite Green Reagent A in 3M sulfuric acid, 3x3ml7. Malachite Green Reagent B (malachite green oxalate and polyvinyl alcohol), 3x3mlThe protocol presented is for a 96-well format. Materials provided are sufficient for two 96-well microplates or equivalent. Storage and Stability:*Store Coupling Phosphatase 1 and UDP at at -20°C. Store other components at RT. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Glycosyltransferase Activity, BioAssay Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glycosyltransferase Activity, BioAssay Kit产品后将其置于RT/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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