Glycogen is the primary short term energy storage molecule in animals, synthesized primarily in the liver and muscle. Glycogen is a branched glucose polymer, in a-1,4 linkage, with branching via a-1,6 linkage. Abnormal ability to utilize glycogen is found in diabetes and in several genetic glycogen storage diseases. The Glycgogen bioassay kit is an easy and convenient assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen to glucose which is then specifically oxidized to produce a product that reacts with OxiRed probe to generate color (lambda-max = 570nm) and fluorescence (Ex 535/Em 587). The assay can detect glycogen 0.0004 to 2mg/ml.Kit Components:Hydrolysis Buffer 1x25mlDevelopment Buffer 1x25mlOxiRed Probe lyophilizedDMSO (anhydrous) 1x0.4mlHydrolysis Enzyme Mix 1x1vialDevelopment Enzyme Mix lyophilizedGlycogen Standard (2.0mg/ml) 1x100ul.Storage and Stability:Store the kit at -20°C, protect from light and moisture. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
