- 中文名称
Glycerol Assay Kit, BioAssay
- 英文名字
- Glycerol Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- G8145-10
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Glycerol [Glycerin or Glycerine, C3H5(OH)3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by- product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide Applications:. Glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex=585/530nm is directly proportional to glycerol concentration in the sample.Key Features:Sensitive and accurate. Use as little as 10ul samples. Linear detection range in 96-well plate: 10 to 1000uM (92 ug/dL to 9.2 mg/dL) glycerol for Colorimetric Assay:s and 2 to 50uM for Fluorometric Assay:s.Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays.Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.Applications:Direct Assays: glycerol in biological samples (e.g. serum and plasma).Drug Discovery/Pharmacology: effects of drugs on glycerol metabolism.Food and Beverages: glycerol in food, beverages, pharmaceutical formulations etc.Kit Contents:Assay Buffer: 24ml Enzyme Mix: 500ul ATP: 250ulDye Reagent: 220ul Standard: 100ul 100mM GlycerolStorage conditions. The kit is shipped on dry ice. Store Assay Buffer at 4°C and other reagents at -20°C. Shelf life of 6 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Colorimetric Procedure:Note: SH-group containing reagents (e.g. mercaptoethanol, DTT) may interfere with this assay and should be avoided in sample preparation.1. Equilibrate all components to room temperature. Keep thawed Enzyme Mix in a refrigerator or on ice. Dilute standard in distilled water as follows (diluted standards can be used for future assays when stored refrigerated).No STD+H2O Vol (ul) Glycerol (mM)1 10ul+990ul 1000 1.02 6ul+994ul 1000 0.63 3ul+997ul 1000 0.34 0ul+1000ul 1000 0Transfer 10ul standards and 10ul samples into separate wells of a clear 96-well plate.2. For each reaction well, mix 100ul Assay Buffer, 2ul Enzyme Mix, 1ul ATP and 1ul Dye Reagent in a clean tube. This Working Reagent should be used on the same day of preparation. Transfer 100ul Working Reagent into each reaction well. Tap plate to mix.3. Incubate 20 min at room temperature. Read optical density at 570nm (550-585nm). Note: if the Sample OD is higher than the Standard OD at 1.0mM, dilute sample in water and repeat the assay. Multiply result by the dilution factor.Calculation:Subtract blank OD (water, #4) from the standard OD values and plot the OD against standard concentrations. Determine the slope using linear regression fitting. The glycerol concentration of Sample is calculated as[Glycerol]=ODSAMPLE–ODH2OSlope(mM)ODSAMPLE and ODH2O are optical density values of the sample and water. Conversions: 1mM glycerol equals 9.2 mg/dL, 92 ppm.Fluorometric Procedure:For Fluorometric Assays, the linear detection range is 2 to 50uM glycerol. Mix 10ul 100mM Standard with 990ul H2O (final 1mM). No 1mM STD+H2O Vol (ul) Glycerol (mM)1 50ul+950ul 1000 0.0502 30ul+970ul 1000 0.0303 15ul+985ul 1000 0.0154 0ul +1000ul 1000 0Dilute standards as above. Transfer 10ul standards and 10ul samples into separate wells of a black 96-well plate. Add 100ul Working Reagent (see Colorimetric Procedure:). Tap plate to mix. Incubate 20 min at room temperature and read fluorescence at lex=530nm and lem=585nm. The glycerol concentration of Sample is calculated as[Glycerol] =FSAMPLE–FH2OSlope(mM)Materials Required, But Not Provided:Pipeting devices, centrifuge tubes, Clear flat-bottom 96-well plates, black 96-well plates (e.g. Corning Costar) and plate reader.
- 产品特点
- 针对Glycerol Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glycerol Assay Kit, BioAssay产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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