- 中文名称
Glutathione, Total, Colorimetric Assay Kit, BioAssay
- 英文名字
- Glutathione, Total, Colorimetric Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- G8135-29
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Glutathione (gamma-glutamyl-cysteinylglycine or GSH) is a naturally occurring tripeptide whose nucleophilic and reducing properties play a central role in metabolic pathways, as well as in the antioxidant system of most aerobic cells. GSH is required as a coenzyme by a variety of enzymes including glutathione peroxidase, glutathione S-transferase and thioltransferase. GSH also plays a major role in drug metabolism, calcium metabolism, the gamma-glutamyl cycle, blood platelet and cell membrane functions. GSH is crucial to a variety of life processes, including the detoxification of xenobiotics, maintenance of the –SH level of proteins, thiol-disulfide exchange, removal of hydroperoxides and free radicals, and amino acid transport across membranes. Physiological values for the concentration of intracellular GSH generally range from 1-10mM.Although many methods have been described for the assay of GSH, most of the reliable ones have been labor intensive.Principle of the Assay:This kit employs a kinetic enzymatic recycling assay, based on the oxidation of GSH by 5,5’- dithiobis(2-nitrobenzoic acid), [DTNB] to measure the total glutathione (tGSH) content of biological samples. The included glutathione standards or treated samples are added to the microtiter plate wells, followed by DTNB and glutathione reductase. Addition of NADPH2 to the wells initiates the progressive reduction of DTNB by GSH, causing a color increase that is monitored at 405nm. The rate of color change, typically followed over a 4 minute time period, is proportional to the tGSH concentration. Consequently, the concentration of tGSH in unknown samples may be determined by reference to the standard curve. GSH reacts with DTNB to produce a colored ion, which absorbs light at 405nm, and a mixed disulfide. This disulfide reacts with further quantities of GSH present to liberate another ion and GSSG. GSSG is reduced enzymatically to GSH which then re-enters the cycle. By adapting the above assay process into a 96 well microtiter plate format, it is now possible to readily measure total GSH (tGSH) in a large number of samples at one time. Since GSSG represents only a small percentage of total acid-solution free glutathione, the resulting values for tGSH (which encompasses both GSH and GSSG) are expressed in units of GSH equivalents.Storage and Stability:Store components as indicated. Kit components are stable for 6-12 months with proper handling and storage.G8135-29B: RTG8135-29G: RTG8135-29H: RTG8135-29D: 4°CG8135-29E: 4°CG8135-29F: 4°CG8135-29A: -20°CG8135-29C: -20°C
- 产品特点
- 针对Glutathione, Total, Colorimetric Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glutathione, Total, Colorimetric Assay Kit, BioAssay产品后将其置于RT/4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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