- 中文名称
Glutathione Reductase, Colorimetric Kit, BioAssay
- 英文名字
- Glutathione Reductase, Colorimetric Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- G8127-20
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Glutathione reductase (GR, EC 1.6.4.2) is a ubiquitous enzyme, which catalyzes the reduction of oxidized glutathione (GSSG) to glutathione (GSH). Glutathione reductase is essential for the glutathione redox cycle that maintains adequate levels of reduced cellular GSH. GSH serves as an antioxidant, reacting with free radicals and organic peroxides, in amino acid transport, and as a substrate for the glutathione peroxidases and glutathione Stransferases in the detoxification of organic peroxides and metabolism of xenobiotics, respectively.1This homodimeric enzyme is a member of the family of flavoprotein disulfide oxidoreductases. Each subunit has four domains; beginning at the N-terminus: an FAD-binding domain, an NADPH-binding domain, a central domain, and an interface domain. The active site of GR is at the dimeric interface. Since the GSSG binding site is composed of residues from both subunits, only the dimeric form is active.2 Glutathione ReductaseGSSG + NADPH + H+ -----------------------------------> 2GSH + NADP+Oxidized glutathione is reduced by a multi-step reaction in which GR is initially reduced by NADPH forming a semiquinone of FAD, a sulfur radical and a thiol. The reduced GR (GRred) reacts with a molecule of GSSG, resulting in a disulfide interchange, which produces a molecule of GSH and the GRred-SG complex. An electron rearrangement in GRred-SG results in a second disulfide interchange, splitting off the second molecule of GSH and restoring the GR to the oxidized form.3 Table1 lists some properties of glutathione reductase.SourceMol. Weight|Km (uM)|Reference|NADPHGSSG|Humerythrocytes100,0008.5654Horse liver107,0008.8595Calf liver 100,000211016Rat liver100,0008.2266Yeast118,0003.8553Principle of the Assay:This assay is based on the oxidation of NADPH to NADP+ catalyzed by a limiting concentration of glutathione reductase. One GR activity unit is defined as the amount of enzyme catalyzing the reduction of one micromole of GSSG per minute at pH 7.6 and 25°C. As shown in the above reaction, one molecule of NADPH is consumed for each molecule of GSSG reduced. Therefore, the reduction of GSSG is determined indirectly by the measurement of the consumption of NADPH, as demonstrated by a decrease in absorbance at 340nm (A340) as a function of time.Reagents:1. NADPH: Lyophilyzed solution containing NADPH, Tris, manitol. 6 vials2. GSSG: Oxidized Glutathione in K•PO4. Buffer containing EDTA. pH 7.53. K•PO4: Potassium Phosphate Buffer, pH 7.5, 20ml4. Diluent Buffer: Potassium Buffer containing BSA/EDTA
- 产品特点
- 针对Glutathione Reductase, Colorimetric Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glutathione Reductase, Colorimetric Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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