- 中文名称
Glutathione Peroxidase 1, Human, BioAssay ELISA Kit (GPX1, GPx-1, GSHPx-1, Cellular Glutathione Peroxidase)
- 英文名字
- Glutathione Peroxidase 1, Human, BioAssay ELISA Kit (GPX1, GPx-1, GSHPx-1, Cellular Glutathione Peroxidase)
- 供应商
- USBiological
- 产品货号
- G8127-11M
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Glutathione peroxidases (GPXs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic /mitochondrial GPX1 (cGPX) is a selenium-dependent enzyme, first of the GPX family to be discovered. GPX2, also known as gastrointestinal GPX (GI-GPX), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPX (pGPX or GPX3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPX4 (PH-GPX) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPX family members, except for the recently described Cys containing GPX3 and epididymis-specific secretory GPX (eGPX or GPX5) isoforms, possess selenocysteine at the active site. Because GPX1 appears to have a major role in the prevention of oxidative stress, it may also be an important antiatherogenic enzyme. In mice, GPX1 deficiency results in abnormal vascular and cardiac function and structure. From a clinical perspective, there is a report suggesting that low erythrocyte GPX1 activity identifies patients with coronary artery disease who are at the high risk for cardiovascular events and that measurement of GPX1 activity provides additional information on risk and might be useful in identifying patients who would benefit from preventive antioxidant treatment.Intended Use:Human Glutathione Peroxidase-1 (human GPX1) ELISA kit is to be used for the in vitro quantitative determination of human GPX1 in human serum, human plasma, human RBC lysate, cell lysate and buffered solution. The assay will recognize both native and recombinant human GPX1. This kit has been configured for research use only and is not to be used in diagnostic procedures.Sensitivity:The minimal detectable dose of human GPX1 was calculated to be 1.56ng/ml, by subtracting two standard deviations from the mean of 10 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.Specificity:The following substances were tested and found to have no crossreactivity: human GPX3, human GPX4, Rat GPX1 and mouse GPX1.Test Principle:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human GPX1. Samples are pippetted into these wells. Nonbound GPX1 and other components of the sample should be removed by washing, then biotin-conjugated monoclonal antibody specific to GPX1 added. In order to quantitatively determine the amount of GPX1 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured GPX1.Kit Components:G8127-11M1 - Microplate, 1x96 wellsG8127-11M2 - Incubation Buffer, 1x30mlG8127-11M3 - Wash Buffer (20X), 2x25mlG8127-11M4 - Standard, 1x1 vialG8127-11M5 - Standard/Sample Dilution Buffer, 1x25mlG8127-11M6 - Secondary Antibody, 1x1 vialG8127-11M7 - Avidin-HRP (100X), 1x150ulG8127-11M8 - Secondary Ab/Avidin-HRP Dilution Buffer, 1x25mlG8127-11M9 - TMB Substrate, 1x15mlG8127-11M10 - Stop Solution, 1x15mlStorage and Stability:Store components at 4°C. Any unused reconstituted standard should be discarded or frozen at -70°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Glutathione Peroxidase 1, Human, BioAssay ELISA Kit (GPX1, GPx-1, GSHPx-1, Cellular Glutathione Peroxidase)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glutathione Peroxidase 1, Human, BioAssay ELISA Kit (GPX1, GPx-1, GSHPx-1, Cellular Glutathione Peroxidase)产品后将其置于4°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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