- 中文名称
GFAP, BioAssay ELISA Kit (Glial Fibrillary Acidic Protein) (Human)
- 英文名字
- GFAP, BioAssay ELISA Kit (Glial Fibrillary Acidic Protein) (Human)
- 供应商
- USBiological
- 产品货号
- G2032-29
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use:The Human GFAP BioAssay ELISA is a biotin-labeled-antibody-based sandwich enzyme immunoassay for the quantitative measurement of human GFAP in serum, CSF, plasma and tissue culture medium. It is intended for in vitro and research use only.Features:• The total assay time is less than five hours.• The kit measures total serum, CSF, or plasma GFAP.• Quality Controls are human serum based. No animal sera are used.• Standard is purified native protein basedSummary:Glial Fibrillary Acidic Protein (GFAP), as a member of the cytoskeletal protein family, is the principal 8-9nm intermediate filament in mature astrocytes of the central nervous system (CNS). GFAP is a monomeric molecule with a molecular mass between 40 and 53kD and an isoelectric point between 5.7 and 5.8. GFAP is a highly brain-specific protein that is not found outside the CNS. Findings have shown that while GFAP is released into the blood very soon after traumatic brain injury (TBI) based on its severity, GFAP is not released after even multiple traumae in the absence of brain injury. In the CNS, following injury, either as a result of trauma, disease, genetic disorders, or chemical insult, astrocytes become reactive and respond in a typical manner, termed astrogliosis. Astrogliosis is characterized by the rapid synthesis of GFAP. GFAP normally increases with age and there is a wide variation in the collection and processing of human brain tissue. Thanks to its high brain specificity and early release from the CNS after TBI, GFAP might be a suitable marker for early diagnostics.Test Principle:In Human GFAP ELISA calibrators or samples are incubated with a rabbit polyclonal anti-human GFAP antibody coated in microtiter wells. After two hours of incubation and a washing, biotin-labeled monoclonal anti-human GFAP antibody is added and incubated with captured GFAP. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour of incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by the addition of acidic solution. The absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of GFAP. A standard curve is constructed by plotting absorbance values versus GFAP concentrations of calibrators, and concentrations of unknown samples are determined using this standard curve.Kit Components:G2032-29A: Microtiter Strips, coated with capture polyclonal antibody, sealed, 1x96 wells*G2032-29B: Human GFAP Master Calibrator, 1x1 vial*G2032-29C: Quality Control: High, 6.3-11.8ng/ml, 1x1 vial*G2032-29D: Quality Control: Low, 1.4-2.6ng/ml, 1x1 vialG2032-29E: Pab (Biotin), 1x13mlG2032-29F: Streptavidin (HRP), 1x13mlG2032-29G: Standard Diluent, 1x9mlG2032-29H: Dilution Buffer, 1x13mlG2032-29J: Wash Solution, 10X, 1x100mlG2032-29K: TMB Substrate Solution, 1x13mlG2032-29L: Stop Solution, H2SO4, 1x13mlStorage and Stability:Store at 4°C. Stable for 6 months after receipt at 4°C.*Note: Store G2032-29B, G2032-29C and G2032-29D at 4°C as a lyophilized powder. Upon reconstitution store at -20°C. Stable for 3 months at -20ºC.
- 产品特点
- 针对GFAP, BioAssay ELISA Kit (Glial Fibrillary Acidic Protein) (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到GFAP, BioAssay ELISA Kit (Glial Fibrillary Acidic Protein) (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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