- 中文名称
Fibroblast Growth Factor, Basic, Human (FGFb) BioAssay ELISA Kit
- 英文名字
- Fibroblast Growth Factor, Basic, Human (FGFb) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- F4210-19
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- This Human Fibroblast Growth Factor basic (FGF-b) ELISA kit is to be used for the quantitative determination of FGF basic in human serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18kD isoform and recombinant FGF basic-146 and FGF basic-157.Sensitivity: <15.6pg/mlRange: 15.6-1000pg/mlIncubation Time: 4 hours, RTSimple: Pre-coated strip-well plates. Liquid stable conjugate, chromogen and stop reagents.Sample size: 50ulAccuracy: Inter-assay CV: <6.7%Intra-assay CV: <5.0%Principle of the Method:This human FGF basic kit is a solid phase sandwich ELISA Assay. A monoclonal antibody specific for human FGF basic has been coated onto the wells of the microtiter strips provided. Samples, including standards of known human FGF-b content, control specimens, and unknowns, are pipetted into these wells. During the first incubation, the FGF-b antigen binds to the immobilized (capture) antibody on one site. After washing, a biotinylated monoclonal antibody specific for FGF-b is added. During the second incubation, this antibody binds to the immobilized FGF-b captured during the first incubation. After removal of excess second antibody, Streptavidin-Peroxidase (enzyme) is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of FGF-b present in the original specimen.Kit Components:F4210-19A: Microtiter Plate, 1x96wells. FGFb antibody-coated wells.F4210-19B: Standard 2x1vial. F4210-19C: Standard Diluent, 1x25ml. Contains 0.1% sodium azide.F4210-19D: FGFb (Biotin) Pab, 1x11ml. Contains 0.1% sodium azide.F4210-19E: Streptavidin (HRP), 100X, 1x125ul. Contains 3.3mM thymol.F4210-19F: Streptavidin-HRP Diluent, 1x25ml. Contains 3.3mM thymol.F4210-19G: Wash Buffer Concentrate, 25X, 1x100ml.F4210-19H: TMB, 1x25ml.F4210-19J: Stop Solution, 1x25ml.Storage and Stability:Store all components at 4°C. Stable for at 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Fibroblast Growth Factor, Basic, Human (FGFb) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Fibroblast Growth Factor, Basic, Human (FGFb) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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